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CaMKII Rabbit mAb (A0198)

Publications (8) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CaMKII Rabbit mAb (A0198)

Western blot analysis of various lysates using CaMKII Rabbit mAb (A0198) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Western blot - CaMKII Rabbit mAb (A0198)

Western blot analysis of various lysates using CaMKII Rabbit mAb (A0198) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - CaMKII Rabbit mAb (A0198)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using CaMKII Rabbit mAb (A0198) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Overview

Product nameCaMKII Rabbit mAb
Catalog No.A0198
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1814
The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Alternative splicing results in multiple transcript variants.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 200-300 of human CaMKII (NP_741960.1).
SequenceGVILYILLVGYPPFWDEDQHRLYQQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPSKRITAAEALKHPWISHRSTVASCMHRQETVDCLKKFNARRKLK
Gene ID
Swiss Prot
SynonymsCAM2; CAMK2; CAMKB; MRD54; CaMKIIbeta; CaMKII
Calculated MW73kDa
Observed MW50kDa/60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IHC-P 1:200 - 1:800
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesSH-SY5Y, U-251MG, Mouse brain, Rat brain, C6
Cellular locationCytoplasm, Cytoplasmic side, Peripheral membrane protein, Sarcoplasmic reticulum membrane, centrosome, cytoskeleton, microtubule organizing center.
Customer validation

WB(Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - CaMKII Rabbit mAb (A0198)}

Western blot - CaMKII Rabbit mAb (A0198)

Western blot analysis of various lysates using CaMKII Rabbit mAb (A0198) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Western blot - CaMKII Rabbit mAb (A0198)}

Western blot - CaMKII Rabbit mAb (A0198)

Western blot analysis of various lysates using CaMKII Rabbit mAb (A0198) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - CaMKII Rabbit mAb (A0198)}

Immunohistochemistry - CaMKII Rabbit mAb (A0198)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using CaMKII Rabbit mAb (A0198) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A0198? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)