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Cation-independent M6PR (IGF2R) Rabbit pAb (A13022)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Cation-independent M6PR (IGF2R) Rabbit pAb (A13022)

Western blot analysis of lysates from HepG2 cells, using Cation-independent M6PR (IGF2R) Rabbit pAb (A13022) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - Cation-independent M6PR (IGF2R) Rabbit pAb (A13022)

Immunofluorescence analysis of C6 cells using Cation-independent M6PR (IGF2R) Rabbit pAb (A13022) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Cation-independent M6PR (IGF2R) Rabbit pAb (A13022)

Immunofluorescence analysis of HeLa cells using Cation-independent M6PR (IGF2R) Rabbit pAb (A13022) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Cation-independent M6PR (IGF2R) Rabbit pAb (A13022)

Immunofluorescence analysis of NIH/3T3 cells using Cation-independent M6PR (IGF2R) Rabbit pAb (A13022) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameCation-independent M6PR (IGF2R) Rabbit pAb
Catalog No.A13022
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate. The binding sites for each ligand are located on different segments of the protein. This receptor has various functions, including in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. Mutation or loss of heterozygosity of this gene has been association with risk of hepatocellular carcinoma. The orthologous mouse gene is imprinted and shows exclusive expression from the maternal allele; however, imprinting of the human gene may be polymorphic, as only a minority of individuals showed biased expression from the maternal allele (PMID:8267611).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 2327-2491 of human Cation-independent M6PR (Cation-independent M6PR (IGF2R)) (NP_000867.2).
SequenceYKKERRETVISKLTTCCRRSSNVSYKYSKVNKEEETDENETEWLMEEIQLPPPRQGKEGQENGHITTKSVKALSSLHGDDQDSEDEVLTIPEVKVHSGRGAGAESSHPVRNAQSNALQEREDDRVGLVRGEKARKGKSSSAQQKTVSSTKLVSFHDDSDEDLLHI
Gene ID
Swiss Prot
SynonymsMPR1; MPRI; CD222; CIMPR; M6P-R; MPR300; CI-M6PR; MPR 300; M6P/IGF2R; Cation-independent M6PR (IGF2R)
Calculated MW274kDa
Observed MW274kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHepG2
Cellular locationLysosome membrane, Single-pass type I membrane protein
Customer validation

WB(Mus musculus)

Documents

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ABclonal:Western blot - Cation-independent M6PR (IGF2R) Rabbit pAb (A13022)}

Western blot - Cation-independent M6PR (IGF2R) Rabbit pAb (A13022)

Western blot analysis of lysates from HepG2 cells, using Cation-independent M6PR (IGF2R) Rabbit pAb (A13022) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunofluorescence - Cation-independent M6PR (IGF2R) Rabbit pAb (A13022)}

Immunofluorescence - Cation-independent M6PR (IGF2R) Rabbit pAb (A13022)

Immunofluorescence analysis of C6 cells using Cation-independent M6PR (IGF2R) Rabbit pAb (A13022) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Cation-independent M6PR (IGF2R) Rabbit pAb (A13022)}

Immunofluorescence - Cation-independent M6PR (IGF2R) Rabbit pAb (A13022)

Immunofluorescence analysis of HeLa cells using Cation-independent M6PR (IGF2R) Rabbit pAb (A13022) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Cation-independent M6PR (IGF2R) Rabbit pAb (A13022)}

Immunofluorescence - Cation-independent M6PR (IGF2R) Rabbit pAb (A13022)

Immunofluorescence analysis of NIH/3T3 cells using Cation-independent M6PR (IGF2R) Rabbit pAb (A13022) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (3)

재조합 단백질 (1)

Secondary Antibodies (26)