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Cleaved PARP (Asp214) Rabbit pAb (A22535)

Publications (4) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Western blot - Cleaved PARP (Asp214) Rabbit pAb (A22535)

Western blot analysis of lysates from NIH/3T3 cells, using Cleaved PARP (Asp214) Rabbit pAb (A22535) at 1:400 dilution.NIH/3T3 cells were treated by staurosporine(1 uM) for 3 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

Overview

Product nameCleaved PARP (Asp214) Rabbit pAb
Catalog No.A22535
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables NAD+ ADP-ribosyltransferase activity; chromatin binding activity; and protein ADP-ribosylase activity. Involved in negative regulation of telomere maintenance via telomere lengthening; positive regulation of single strand break repair; and voluntary musculoskeletal movement. Acts upstream of or within several processes, including DNA metabolic process; behavioral response to cocaine; and protein poly-ADP-ribosylation. Located in cytoplasm; nucleolus; and nucleoplasm. Part of protein-containing complex. Is expressed in several structures, including Peyer's patch; brain; gut; immune system; and retina. Human ortholog(s) of this gene implicated in several diseases, including arthritis; bacterial meningitis; neurodegenerative disease (multiple); stomach carcinoma; and type 2 diabetes mellitus. Orthologous to human PARP1 (poly(ADP-ribose) polymerase 1).
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 201-300 of Mouse PARP (Asp214) (NP_031441.2).
SequenceAIKNEGKRKGDEVDGTDEVAKKKSKKGKDKDSSKLEKALKAQNELIWNIKDELKKACSTNDLKELLIFNQQQVPSGESAILDRVADGMAFGALLPCKECS
Gene ID
Swiss Prot
SynonymsPARP; PPOL; ARTD1; Adprp; Adprt1; msPARP; parp-1; sPARP-1; 5830444G22Rik; Cleaved PARP (Asp214)
Calculated MW113kDa
Observed MW89kDa
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesNIH/3T3 treated by staurosporine
Cellular locationcytosol, mitochondrion, nuclear body, nuclear envelope, nuclear replication fork, nucleolus
Customer validation

WB(Mus musculus, Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Cleaved PARP (Asp214) Rabbit pAb (A22535)}

    Western blot - Cleaved PARP (Asp214) Rabbit pAb (A22535)

    Western blot analysis of lysates from NIH/3T3 cells, using Cleaved PARP (Asp214) Rabbit pAb (A22535) at 1:400 dilution.NIH/3T3 cells were treated by staurosporine(1 uM) for 3 hour.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A22535? Please let us know so that we can cite the reference in this datasheet.