제품 > 항체 > 다중클론항체(pAb)

Cytokeratin 17 (KRT17) Rabbit pAb (A2175)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of extracts of various cell lines, using Cytokeratin 17 (Cytokeratin 17 (KRT17)) antibody (A2175) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunofluorescence - Cytokeratin 17 (KRT17) Rabbit pAb (A2175)

Immunofluorescence analysis of A-549 cells using Cytokeratin 17 (Cytokeratin 17 (KRT17)) antibody (A2175). Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of HeLa cells using Cytokeratin 17 (Cytokeratin 17 (KRT17)) antibody (A2175) at dilution of 1:200. Blue: DAPI for nuclear staining.

Overview

Product name	Cytokeratin 17 (KRT17) Rabbit pAb
Catalog No.	A2175
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes the type I intermediate filament chain keratin 17, expressed in nail bed, hair follicle, sebaceous glands, and other epidermal appendages. Mutations in this gene lead to Jackson-Lawler type pachyonychia congenita and steatocystoma multiplex.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 133-432 of human Cytokeratin 17 (Cytokeratin 17 (KRT17)) (NP_000413.1).
Sequence	QYYRTIEELQNKILTATVDNANILLQIDNARLAADDFRTKFETEQALRLSVEADINGLRRVLDELTLARADLEMQIENLKEELAYLKKNHEEEMNALRGQVGGEINVEMDAAPGVDLSRILNEMRDQYEKMAEKNRKDAEDWFFSKTEELNREVATNSELVQSGKSEISELRRTMQALEIELQSQLSMKASLEGNLAETENRYCVQLSQIQGLIGSVEEQLAQLRCEMEQQNQEYKILLDVKTRLEQEIATYRRLLEGEDAHLTQYKKEPVTTRQVRTIVEEVQDGKVISSREQVHQTTR
Gene ID
Swiss Prot
Synonyms	PC; K17; PC2; 39.1; CK-17; PCHC1
Calculated MW	48kDa
Observed MW	48kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:20 - 1:100
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	HeLa, HepG2, OVCAR3
Cellular location	Cytoplasm

Documents

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Research Area

Cytokeratin 17 (KRT17) Rabbit pAb images

Western blot - Cytokeratin 17 (KRT17) Rabbit pAb (A2175)

Immunofluorescence - Cytokeratin 17 (KRT17) Rabbit pAb (A2175)

Immunofluorescence analysis of A-549 cells using Cytokeratin 17 (Cytokeratin 17 (KRT17)) antibody (A2175). Blue: DAPI for nuclear staining.

Immunofluorescence - Cytokeratin 17 (KRT17) Rabbit pAb (A2175)

Confocal immunofluorescence analysis of HeLa cells using Cytokeratin 17 (Cytokeratin 17 (KRT17)) antibody (A2175) at dilution of 1:200. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.