제품 > 항체 > 다중클론항체(pAb)

DAB1 Rabbit pAb (A10349)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - DAB1 Rabbit pAb (A10349)

Western blot analysis of various lysates using DAB1 Rabbit pAb (A10349) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - DAB1 Rabbit pAb (A10349)

Immunofluorescence analysis of SH-SY5Y cells using DAB1 Rabbit pAb (A10349) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameDAB1 Rabbit pAb
Catalog No.A10349
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The laminar organization of multiple neuronal types in the cerebral cortex is required for normal cognitive function. In mice, the disabled-1 gene plays a central role in brain development, directing the migration of cortical neurons past previously formed neurons to reach their proper layer. This gene is similar to disabled-1, and the protein encoded by this gene is thought to be a signal transducer that interacts with protein kinase pathways to regulate neuronal positioning in the developing brain.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human DAB1 (NP_066566.3).
SequenceMSTETELQVAVKTSAKKDSRKKGQDRSEATLIKRFKGEGVRYKAKLIGIDEVSAARGDKLCQDSMMKLKGVVAGARSKGEHKQKIFLTISFGGIKIFDEKTGALQHHHAVHEISYIAKDITDHRAFGYVCGKEGNHRFVAIKTAQAAEPVILDLRDLFQLIYELKQREELEKKAQKDKQCEQAVY
Gene ID
Swiss Prot
SynonymsSCA37; DAB1
Calculated MW64kDa
Observed MW57kDa/59kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse small intestine, Rat brain
Cellular locationcytoplasm, cytosol, perinuclear region of cytoplasm.
Customer validation

WB(Mus musculus)

IHC(Rattus norvegicus)

Documents

Certificate of Compliance

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ABclonal:Western blot - DAB1 Rabbit pAb (A10349)}

Western blot - DAB1 Rabbit pAb (A10349)

Western blot analysis of various lysates using DAB1 Rabbit pAb (A10349) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - DAB1 Rabbit pAb (A10349)}

Immunofluorescence - DAB1 Rabbit pAb (A10349)

Immunofluorescence analysis of SH-SY5Y cells using DAB1 Rabbit pAb (A10349) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A10349? Please let us know so that we can cite the reference in this datasheet.

Secondary Antibodies (26)