Western blot - DBI Rabbit pAb (A13293)
Western blot analysis of various lysates using DBI Rabbit pAb (A13293) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot - DBI Rabbit pAb (A13293)
Western blot analysis of various lysates using DBI Rabbit pAb (A13293) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Immunohistochemistry - DBI Rabbit pAb (A13293)
Immunohistochemistry analysis of paraffin-embedded Rat liver using DBI Rabbit pAb (A13293) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - DBI Rabbit pAb (A13293)
Immunohistochemistry analysis of paraffin-embedded Rat ovary using DBI Rabbit pAb (A13293) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunofluorescence - DBI Rabbit pAb (A13293)
Immunofluorescence analysis of A-549 cells using DBI Rabbit pAb (A13293) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence - DBI Rabbit pAb (A13293)
Immunofluorescence analysis of NIH/3T3 cells using DBI Rabbit pAb (A13293) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence - DBI Rabbit pAb (A13293)
Immunofluorescence analysis of PC-12 cells using DBI Rabbit pAb (A13293) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence - DBI Rabbit pAb (A13293)
Immunofluorescence analysis of U2OS cells using DBI Rabbit pAb (A13293) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation - DBI Rabbit pAb (A13293)
Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg DBI antibody (A13293). Western blot was performed from the immunoprecipitate using DBI antibody (A13293) at a dilution of 1:1000.
Immunoprecipitation - DBI Rabbit pAb (A13293)
Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg DBI antibody (A13293). Western blot was performed from the immunoprecipitate using DBI antibody (A13293) at a dilution of 1:1000.
Immunoprecipitation - DBI Rabbit pAb (A13293)
Immunoprecipitation of DBI in 300 µg extracts from HeLa cells using 3 µg [KO Validated] DBI Rabbit pAb (A13293). Western blot analysis was performed using [KO Validated] DBI Rabbit pAb (A13293) at 1:1000 dilution.