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DBI Rabbit pAb (A13293)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - DBI Rabbit pAb (A13293)

Western blot analysis of various lysates using DBI Rabbit pAb (A13293) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - DBI Rabbit pAb (A13293)

Western blot analysis of various lysates using DBI Rabbit pAb (A13293) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - DBI Rabbit pAb (A13293)

Immunohistochemistry analysis of paraffin-embedded Rat liver using DBI Rabbit pAb (A13293) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - DBI Rabbit pAb (A13293)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using DBI Rabbit pAb (A13293) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - DBI Rabbit pAb (A13293)

Immunofluorescence analysis of A-549 cells using DBI Rabbit pAb (A13293) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - DBI Rabbit pAb (A13293)

Immunofluorescence analysis of NIH/3T3 cells using DBI Rabbit pAb (A13293) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - DBI Rabbit pAb (A13293)

Immunofluorescence analysis of PC-12 cells using DBI Rabbit pAb (A13293) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - DBI Rabbit pAb (A13293)

Immunofluorescence analysis of U2OS cells using DBI Rabbit pAb (A13293) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - DBI Rabbit pAb (A13293)

Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg DBI antibody (A13293). Western blot was performed from the immunoprecipitate using DBI antibody (A13293) at a dilution of 1:1000.

ABclonal:Immunoprecipitation - DBI Rabbit pAb (A13293)

Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg DBI antibody (A13293). Western blot was performed from the immunoprecipitate using DBI antibody (A13293) at a dilution of 1:1000.

ABclonal:Immunoprecipitation - DBI Rabbit pAb (A13293)

Immunoprecipitation of DBI in 300 µg extracts from HeLa cells using 3 µg [KO Validated] DBI Rabbit pAb (A13293). Western blot analysis was performed using [KO Validated] DBI Rabbit pAb (A13293) at 1:1000 dilution.

Overview

Product nameDBI Rabbit pAb
Catalog No.A13293
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. The protein is conserved from yeast to mammals, with the most highly conserved domain consisting of seven contiguous residues that constitute the hydrophobic binding site for medium- and long-chain acyl-Coenzyme A esters. Diazepam binding inhibitor is also known to mediate the feedback regulation of pancreatic secretion and the postprandial release of cholecystokinin, in addition to its role as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes located on chromosomes 6, 8 and 16 have been identified. Multiple transcript variants encoding different isoforms have been described for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-104 of human DBI (NP_001171513.1).
SequenceMWGDLWLLPPASANPGTGTEAEFEKAAEEVRHLKTKPSDEEMLFIYGHYKQATVGDINTERPGMLDFTGKAKWDAWNELKGTSKEDAMKAYINKVEELKKKYGI
Gene ID
Swiss Prot
SynonymsEP; ACBP; ACBD1; CCK-RP; DBI
Calculated MW10kDa
Observed MW11kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, HepG2, U-87MG, Rat testis, Rat ovary
Cellular locationEndoplasmic reticulum, Golgi apparatus.
Customer validation

WB(Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - DBI Rabbit pAb (A13293)}

Western blot - DBI Rabbit pAb (A13293)

Western blot analysis of various lysates using DBI Rabbit pAb (A13293) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - DBI Rabbit pAb (A13293)}

Western blot - DBI Rabbit pAb (A13293)

Western blot analysis of various lysates using DBI Rabbit pAb (A13293) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - DBI Rabbit pAb (A13293)}

Immunohistochemistry - DBI Rabbit pAb (A13293)

Immunohistochemistry analysis of paraffin-embedded Rat liver using DBI Rabbit pAb (A13293) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - DBI Rabbit pAb (A13293)}

Immunohistochemistry - DBI Rabbit pAb (A13293)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using DBI Rabbit pAb (A13293) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - DBI Rabbit pAb (A13293)}

Immunofluorescence - DBI Rabbit pAb (A13293)

Immunofluorescence analysis of A-549 cells using DBI Rabbit pAb (A13293) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - DBI Rabbit pAb (A13293)}

Immunofluorescence - DBI Rabbit pAb (A13293)

Immunofluorescence analysis of NIH/3T3 cells using DBI Rabbit pAb (A13293) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - DBI Rabbit pAb (A13293)}

Immunofluorescence - DBI Rabbit pAb (A13293)

Immunofluorescence analysis of PC-12 cells using DBI Rabbit pAb (A13293) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - DBI Rabbit pAb (A13293)}

Immunofluorescence - DBI Rabbit pAb (A13293)

Immunofluorescence analysis of U2OS cells using DBI Rabbit pAb (A13293) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - DBI Rabbit pAb (A13293)}

Immunoprecipitation - DBI Rabbit pAb (A13293)

Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg DBI antibody (A13293). Western blot was performed from the immunoprecipitate using DBI antibody (A13293) at a dilution of 1:1000.
ABclonal:Immunoprecipitation - DBI Rabbit pAb (A13293)}

Immunoprecipitation - DBI Rabbit pAb (A13293)

Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg DBI antibody (A13293). Western blot was performed from the immunoprecipitate using DBI antibody (A13293) at a dilution of 1:1000.
ABclonal:Immunoprecipitation - DBI Rabbit pAb (A13293)}

Immunoprecipitation - DBI Rabbit pAb (A13293)

Immunoprecipitation of DBI in 300 µg extracts from HeLa cells using 3 µg [KO Validated] DBI Rabbit pAb (A13293). Western blot analysis was performed using [KO Validated] DBI Rabbit pAb (A13293) at 1:1000 dilution.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

Secondary Antibodies (26)