Western blot - DDX5 Rabbit mAb (A11339)
Western blot analysis of various lysates using DDX5 Rabbit mAb (A11339) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry - DDX5 Rabbit mAb (A11339)
Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using DDX5 Rabbit mAb (A11339) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - DDX5 Rabbit mAb (A11339)
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using DDX5 Rabbit mAb (A11339) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - DDX5 Rabbit mAb (A11339)
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using DDX5 Rabbit mAb (A11339) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - DDX5 Rabbit mAb (A11339)
Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using DDX5 Rabbit mAb (A11339) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - DDX5 Rabbit mAb (A11339)
Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using DDX5 Rabbit mAb (A11339) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - DDX5 Rabbit mAb (A11339)
Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using DDX5 Rabbit mAb (A11339) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - DDX5 Rabbit mAb (A11339)
Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using DDX5 Rabbit mAb (A11339) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - DDX5 Rabbit mAb (A11339)
Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using DDX5 Rabbit mAb (A11339) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - DDX5 Rabbit mAb (A11339)
Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using DDX5 Rabbit mAb (A11339) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - DDX5 Rabbit mAb (A11339)
Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using DDX5 Rabbit mAb (A11339) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - DDX5 Rabbit mAb (A11339)
Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using DDX5 Rabbit mAb (A11339) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunofluorescence - DDX5 Rabbit mAb (A11339)
Confocal imaging of U-2 OS cells using DDX5 Rabbit mAb (A11339, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.
Immunofluorescence - DDX5 Rabbit mAb (A11339)
Confocal imaging of Hep G2 cells using DDX5 Rabbit mAb (A11339, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® " +
"488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.
Immunofluorescence - DDX5 Rabbit mAb (A11339)
Confocal imaging of C2C12 cells using DDX5 Rabbit mAb (A11339, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.
Immunoprecipitation - DDX5 Rabbit mAb (A11339)
Immunoprecipitation analysis of 600 μg extracts of Mouse testis using 3 μg DDX5 antibody (A11339). Western blot was performed from the immunoprecipitate using DDX5 (A11339) at a dilution of 1:1000.