제품 > 항체 > 다중클론항체(pAb)

DHX58 Rabbit pAb (A8257)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - DHX58 Rabbit pAb (A8257)

Western blot analysis of various lysates, using DHX58 Rabbit pAb (A8257) at 1:9000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - DHX58 Rabbit pAb (A8257)

Immunofluorescence analysis of U2OS cells using DHX58 antibody (A8257) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameDHX58 Rabbit pAb
Catalog No.A8257
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables double-stranded RNA binding activity; single-stranded RNA binding activity; and zinc ion binding activity. Involved in negative regulation of defense response and negative regulation of type I interferon production. Predicted to be active in cytoplasm.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 389-678 of human DHX58 (NP_077024.2).
SequenceQQQGLQTVDIRAQLLIGAGNSSQSTHMTQRDQQEVIQKFQDGTLNLLVATSVAEEGLDIPHCNVVVRYGLLTNEISMVQARGRARADQSVYAFVATEGSRELKRELINEALETLMEQAVAAVQKMDQAEYQAKIRDLQQAALTKRAAQAAQRENQRQQFPVEHVQLLCINCMVAVGHGSDLRKVEGTHHVNVNPNFSNYYNVSRDPVVINKVFKDWKPGGVISCRNCGEVWGLQMIYKSVKLPVLKVRSMLLETPQGRIQAKKWSRVPFSVPDFDFLQHCAENLSDLSLD
Gene ID
Swiss Prot
SynonymsLGP2; RLR-3; D11LGP2; D11lgp2e
Calculated MW77kDa
Observed MW77kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse spleen, Rat spleen, Rat lung
Cellular locationCytoplasm
Customer validation

WB(Cynoglossus semilaevis, Mus musculus)

Documents

Certificate of Compliance

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    ABclonal:Western blot - DHX58 Rabbit pAb (A8257)}

    Western blot - DHX58 Rabbit pAb (A8257)

    Western blot analysis of various lysates, using DHX58 Rabbit pAb (A8257) at 1:9000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunofluorescence - DHX58 Rabbit pAb (A8257)}

    Immunofluorescence - DHX58 Rabbit pAb (A8257)

    Immunofluorescence analysis of U2OS cells using DHX58 antibody (A8257) at dilution of 1:100. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A8257? Please let us know so that we can cite the reference in this datasheet.