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DLAT Rabbit pAb (A14530)

Review (1)Publications (8) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - DLAT Rabbit pAb (A14530)

Western blot analysis of various lysates, using DLAT Rabbit pAb (A14530) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - DLAT Rabbit pAb (A14530)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using DLAT Rabbit pAb (A14530) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - DLAT Rabbit pAb (A14530)

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using DLAT Rabbit pAb (A14530) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - DLAT Rabbit pAb (A14530)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using DLAT Rabbit pAb (A14530) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - DLAT Rabbit pAb (A14530)

Immunofluorescence analysis of Hep G2 cells using DLAT Rabbit pAb (A14530) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for DLAT Rabbit pAb(A14530)

Overview

Product nameDLAT Rabbit pAb
Catalog No.A14530
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 87-270 of human DLAT (NP_001922.2).
SequenceSLPPHQKVPLPSLSPTMQAGTIARWEKKEGDKINEGDLIAEVETDKATVGFESLEECYMAKILVAEGTRDVPIGAIICITVGKPEDIEAFKNYTLDSSAAPTPQAAPAPTPAATASPPTPSAQAPGSSYPPHMQVLLPALSPTMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEG
Gene ID
Swiss Prot
SynonymsE2; PBC; DLTA; PDCE2; PDC-E2; DLAT
Calculated MW69kDa
Observed MW69kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesK562, HeLa, MCF7
Cellular locationMitochondrion matrix.
Customer validation

(mouse heart cells)

WB(Gallus gallus, Homo sapiens)

IHC(Homo sapiens)

IF(Mus musculus, Homo sapiens)

Cell-based assay(Homo sapiens)

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - DLAT Rabbit pAb (A14530)}

Western blot - DLAT Rabbit pAb (A14530)

Western blot analysis of various lysates, using DLAT Rabbit pAb (A14530) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - DLAT Rabbit pAb (A14530)}

Immunohistochemistry - DLAT Rabbit pAb (A14530)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using DLAT Rabbit pAb (A14530) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - DLAT Rabbit pAb (A14530)}

Immunohistochemistry - DLAT Rabbit pAb (A14530)

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using DLAT Rabbit pAb (A14530) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - DLAT Rabbit pAb (A14530)}

Immunohistochemistry - DLAT Rabbit pAb (A14530)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using DLAT Rabbit pAb (A14530) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - DLAT Rabbit pAb (A14530)}

Immunofluorescence - DLAT Rabbit pAb (A14530)

Immunofluorescence analysis of Hep G2 cells using DLAT Rabbit pAb (A14530) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

Secondary Antibodies (26)