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DLST Rabbit pAb (A13297)

Review (1)Publications (9) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - DLST Rabbit pAb (A13297)

Western blot analysis of various lysates using DLST Rabbit pAb (A13297) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - DLST Rabbit pAb (A13297)

Immunohistochemistry analysis of paraffin-embedded Human liver damage using DLST Rabbit pAb (A13297) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - DLST Rabbit pAb (A13297)

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using DLST Rabbit pAb (A13297) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - DLST Rabbit pAb (A13297)

Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using DLST Rabbit pAb (A13297) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - DLST Rabbit pAb (A13297)

Immunofluorescence analysis of U-2 OS cells using DLST Rabbit pAb (A13297) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for DLST Rabbit pAb(A13297)

Overview

Product nameDLST Rabbit pAb
Catalog No.A13297
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a mitochondrial protein that belongs to the 2-oxoacid dehydrogenase family. This protein is one of the three components (the E2 component) of the 2-oxoglutarate dehydrogenase complex that catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). Alternatively spliced transcript variants have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 150-250 of human DLST (NP_001924.2).
SequencePAKAKPAEAPAAAAPKAEPTAAAVPPPAAPIPTQMPPVPSPSQPPSGKPVSAVKPTVAPPLAEPGAGKGLRSEHREKMNRMRQRIAQRLKEAQNTCAMLTT
Gene ID
Swiss Prot
SynonymsDLTS; KGD2; PGL7; DLST
Calculated MW49kDa
Observed MW49kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:50 - 1:100
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse liver, Mouse kidney, Rat kidney, Rat liver
Cellular locationMitochondrion.
Customer validation

(mouse heart cells)

WB(Gallus gallus, Mus musculus, Oryctolagus cuniculus, Sus scrofa, Homo sapiens)

IHC(Homo sapiens)

ChIP(Homo sapiens)

WB(Homo sapiens)

IP(Sus scrofa)

Documents

Certificate of Compliance

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ABclonal:Western blot - DLST Rabbit pAb (A13297)}

Western blot - DLST Rabbit pAb (A13297)

Western blot analysis of various lysates using DLST Rabbit pAb (A13297) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - DLST Rabbit pAb (A13297)}

Immunohistochemistry - DLST Rabbit pAb (A13297)

Immunohistochemistry analysis of paraffin-embedded Human liver damage using DLST Rabbit pAb (A13297) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - DLST Rabbit pAb (A13297)}

Immunohistochemistry - DLST Rabbit pAb (A13297)

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using DLST Rabbit pAb (A13297) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - DLST Rabbit pAb (A13297)}

Immunohistochemistry - DLST Rabbit pAb (A13297)

Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using DLST Rabbit pAb (A13297) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - DLST Rabbit pAb (A13297)}

Immunofluorescence - DLST Rabbit pAb (A13297)

Immunofluorescence analysis of U-2 OS cells using DLST Rabbit pAb (A13297) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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