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Dopamine beta Hydroxylase Rabbit mAb (A23579)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Dopamine beta Hydroxylase Rabbit mAb (A23579)

Western blot analysis of lysates from SH-SY5Y cells, using Dopamine beta Hydroxylase Rabbit mAb (A23579) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product nameDopamine beta Hydroxylase Rabbit mAb
Catalog No.A23579
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC3095
The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. The encoded protein, expressed in neuroscretory vesicles and chromaffin granules of the adrenal medulla, catalyzes the conversion of dopamine to norepinephrine, which functions as both a hormone and as the main neurotransmitter of the sympathetic nervous system. The enzyme encoded by this gene exists exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. Mutations in this gene cause dopamine beta-hydroxylate deficiency in human patients, characterized by deficits in autonomic and cardiovascular function, including hypotension and ptosis. Polymorphisms in this gene may play a role in a variety of psychiatric disorders.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 100-250 of human Dopamine beta Hydroxylase (NP_000778.3).
SequenceLVVLWTDGDTAYFADAWSDQKGQIHLDPQQDYQLLQVQRTPEGLTLLFKRPFGTCDPKDYLIEDGTVHLVYGILEEPFRSLEAINGSGLQMGLQRVQLLKPNIPEPELPSDACTMEVQAPNIQIPSQETTYWCYIKELPKGFSRHHIIKYE
Gene ID
Swiss Prot
SynonymsDBM; ORTHYP1; Dopamine beta Hydroxylase
Calculated MW69kDa
Observed MW75-80kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesSH-SY5Y
Cellular locationCytoplasmic vesicle, Single-pass type II membrane protein, chromaffin granule lumen, chromaffin granule membrane, secretory vesicle, secretory vesicle lumen, secretory vesicle membrane.

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    ABclonal:Western blot - Dopamine beta Hydroxylase Rabbit mAb (A23579)}

    Western blot - Dopamine beta Hydroxylase Rabbit mAb (A23579)

    Western blot analysis of lysates from SH-SY5Y cells, using Dopamine beta Hydroxylase Rabbit mAb (A23579) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    항체 (2)

    ELISA 키트 (1)

    Secondary Antibodies (26)