제품 > 항체 > 단일클론항체(mAb)

E-Cadherin mouse mAb (A3149)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Immunohistochemistry analysis of paraffin-embedded Human breast tissue using E-Cadherin mouse mAb (A3149) at a dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using E-Cadherin mouse mAb (A3149) at a dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using E-Cadherin mouse mAb (A3149) at a dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human skin tissue using E-Cadherin mouse mAb (A3149) at a dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Overview

Product name	E-Cadherin mouse mAb
Catalog No.	A3149
Host species	Mouse
Purification method	Protein A purified
Isotype	IgG1, Kappa
CloneNo.	AMC0478

Background

This gene encodes E-cadherin, a calcium-dependent cell adhesion molecule that functions in the establishment and maintenance of epithelial cell morphology during embryongenesis and adulthood. The encoded preproprotein undergoes proteolytic processing to generate a mature protein. Targeted mutations disrupting binding of calcium to the encoded protein in mice cause death in utero due to failed blastocyst and trophectoderm formation. This gene is located adjacent to a related cadherin gene on chromosome 8.

Immunogen information

Immunogen	Recombinant protein of mouse E-Cadherin.
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	Um; UVO; Ecad; ARC-1; E-cad; L-CAM; E-Cadherin
Calculated MW	98kDa
Observed MW	Refer to figures

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	IHC-P 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% sodium azide, 0.2% BSA, 40% glycerol, pH7.4.
Key application	Immunohistochemistry
Positive samples
Cellular location	actin cytoskeleton, axon, axon terminus, basolateral plasma membrane, cortical actin cytoskeleton, cytoplasm, cytoplasmic side of plasma membrane, endosome, flotillin complex, glutamatergic synapse, lateral loop, lateral plasma membrane, perinuclear region of cytoplasm, plasma membrane, postsynapse, trans-Golgi network.
Customer validation	WB(Homo sapiens)