제품 > 항체 > 다중클론항체(pAb)

CD324/E-Cadherin Rabbit pAb (A24874)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of various lysates using CD324/E-Cadherin Rabbit pAb (A24874) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): HeLa.
Exposure time: 5s.

Overview

Product name	CD324/E-Cadherin Rabbit pAb
Catalog No.	A24874
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes E-cadherin, a calcium-dependent cell adhesion molecule that functions in the establishment and maintenance of epithelial cell morphology during embryongenesis and adulthood. The encoded preproprotein undergoes proteolytic processing to generate a mature protein. Targeted mutations disrupting binding of calcium to the encoded protein in mice cause death in utero due to failed blastocyst and trophectoderm formation. This gene is located adjacent to a related cadherin gene on chromosome 8.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 157-369 of mouse CD324/E-Cadherin (NP_033994.1).
Sequence	WVIPPISCPENEKGEFPKNLVQIKSNRDKETKVFYSITGQGADKPPVGVFIIERETGWLKVTQPLDREAIAKYILYSHAVSSNGEAVEDPMEIVITVTDQNDNRPEFTQEVFEGSVAEGAVPGTSVMKVSATDADDDVNTYNAAIAYTIVSQDPELPHKNMFTVNRDTGVISVLTSGLDRESYPTYTLVVQAADLQGEGLSTTAKAVITVKD
Gene ID
Swiss Prot
Synonyms	CDH1; Arc-1; CD324; CDHE; ECAD; LCAM; UVO; AA960649; ARC-1; E-cad; Ecad; L-CAM; Um; cadherin-1; CD324/E-Cadherin
Calculated MW	98kDa
Observed MW	135kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	T-47D, Mouse lung
Cellular location	Cell junction, adherens junction, Cell membrane, Endosome, Golgi apparatus, trans-Golgi network
Customer validation	WB(Homo sapiens)