제품 > 항체 > 단일클론항체(mAb)

ABflo® 488 Rabbit anti-Mouse E-Cadherin/CD324 mAb (A26483)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Flow cytometry: 1X10^6 CHO-S cells (negative control, left) and CHO-S (Transfection, right) cells were surface-stained with ABflo® 488 Rabbit anti-Mouse E-Cadherin/CD324 mAb (A26483, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 CHO-S (Transfection) cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Mouse E-Cadherin/CD324 mAb (A26483, 5 μl/Test, right).

Overview

Product name	ABflo® 488 Rabbit anti-Mouse E-Cadherin/CD324 mAb
Catalog No.	A26483
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC68219-ABflo488

Background

This gene encodes E-cadherin, a calcium-dependent cell adhesion molecule that functions in the establishment and maintenance of epithelial cell morphology during embryongenesis and adulthood. The encoded preproprotein undergoes proteolytic processing to generate a mature protein. Targeted mutations disrupting binding of calcium to the encoded protein in mice cause death in utero due to failed blastocyst and trophectoderm formation. This gene is located adjacent to a related cadherin gene on chromosome 8.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 157-369 of mouse E-Cadherin/CD324 (NP_033994.1).
Sequence	DWVIPPISCPENEKGEFPKNLVQIKSNRDKETKVFYSITGQGADKPPVGVFIIERETGWLKVTQPLDREAIAKYILYSHAVSSNGEAVEDPMEIVITVTDQNDNRPEFTQEVFEGSVAEGAVPGTSVMKVSATDADDDVNTYNAAIAYTIVSQDPELPHKNMFTVNRDTGVISVLTSGLDRESYPTYTLVVQAADLQGEGLSTTAKAVITVKD
Gene ID
Swiss Prot
Synonyms	Um; UVO; Ecad; ARC-1; E-cad; L-CAM
Calculated MW	98kDa
Observed MW

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	actin cytoskeleton, axon, axon terminus, basolateral plasma membrane, cortical actin cytoskeleton, cytoplasm, cytoplasmic side of plasma membrane, endosome, flotillin complex, glutamatergic synapse, lateral loop, lateral plasma membrane, perinuclear region of cytoplasm, plasma membrane, postsynapse, trans-Golgi network.
Customer validation	WB(Other)