제품 > 항체 > 다중클론항체(pAb)

EIF3H Rabbit pAb (A13378)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using EIF3H Rabbit pAb (A13378) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - EIF3H Rabbit pAb (A13378)

Immunofluorescence analysis of U2OS cells using EIF3H Rabbit pAb (A13378). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - EIF3H Rabbit pAb (A13378)

Immunoprecipitation analysis of 200 μg extracts of Jurkat cells using 1 μg EIF3H antibody (A13378). Western blot was performed from the immunoprecipitate using EIF3H antibody (A13378) at a dilution of 1:1000.

Overview

Product name	EIF3H Rabbit pAb
Catalog No.	A13378
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Enables deubiquitinase activity. Contributes to translation initiation factor activity. Involved in negative regulation of proteasomal ubiquitin-dependent protein catabolic process and translational initiation. Located in extracellular exosome and membrane. Part of eukaryotic translation initiation factor 3 complex. Implicated in breast cancer; prostate cancer; and prostate carcinoma. Biomarker of prostate cancer.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 80-340 of human EIF3H (NP_003747.1).
Sequence	NCFPFPQHTEDDADFDEVQYQMEMMRSLRHVNIDHLHVGWYQSTYYGSFVTRALLDSQFSYQHAIEESVVLIYDPIKTAQGSLSLKAYRLTPKLMEVCKEKDFSPEALKKANITFEYMFEEVPIVIKNSHLINVLMWELEKKSAVADKHELLSLASSNHLGKNLQLLMDRVDEMSQDIVKYNTYMRNTSKQQQQKHQYQQRRQQENMQRQSRGEPPLPEEDLSKLFKPPQPPARMDSLLIAGQINTYCQNIKEFTAQNLGK
Gene ID
Swiss Prot
Synonyms	EIF3S3; eIF3-p40; eIF3-gamma; EIF3H
Calculated MW	40kDa
Observed MW	40kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:200 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunoprecipitation Immunofluorescence
Positive samples	HL-60, HT-29, NIH/3T3, HeLa, Jurkat, Mouse spleen, Mouse testis, Mouse heart, Rat spleen
Cellular location	Cytoplasm.

Documents

Certificate of Compliance

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Lot number

Research Area

연구후성유전학 & 핵 신호전달RNA 결합

EIF3H Rabbit pAb images

Western blot - EIF3H Rabbit pAb (A13378)

Immunofluorescence - EIF3H Rabbit pAb (A13378)

Immunofluorescence analysis of U2OS cells using EIF3H Rabbit pAb (A13378). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunoprecipitation - EIF3H Rabbit pAb (A13378)

* For research use only. Not for therapeutic or diagnostic purposes.