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ENO3 Rabbit pAb (A16371)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ENO3 Rabbit pAb (A16371)

Western blot analysis of extracts of various cell lines, using ENO3 Rabbit pAb (A16371) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - ENO3 Rabbit pAb (A16371)

Immunofluorescence analysis of HeLa cells using ENO3 antibody (A16371) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ENO3 Rabbit pAb (A16371)

Immunofluorescence analysis of NIH/3T3 cells using ENO3 antibody (A16371) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ENO3 Rabbit pAb (A16371)

Immunofluorescence analysis of PC-12 cells using ENO3 antibody (A16371) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameENO3 Rabbit pAb
Catalog No.A16371
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme is found in skeletal muscle cells in the adult where it may play a role in muscle development and regeneration. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have be associated glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been described.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human ENO3 (NP_001967.3).
SequenceMAMQKIFAREILDSRGNPTVEVDLHTAKGRFRAAVPSGASTGIYEALELRDGDKGRYLGKGVLKAVENINNTLGPALLQKKLSVVDQEKVDKFMIELDGTENKSKFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGNPDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMRIGAEVYH
Gene ID
Swiss Prot
SynonymsMSE; GSD13
Calculated MW47kDa
Observed MW47kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse heart, Mouse skeletal muscle, Rat heart, Rat skeletal muscle
Cellular locationCytoplasm

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ABclonal:Western blot - ENO3 Rabbit pAb (A16371)}

Western blot - ENO3 Rabbit pAb (A16371)

Western blot analysis of extracts of various cell lines, using ENO3 Rabbit pAb (A16371) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - ENO3 Rabbit pAb (A16371)}

Immunofluorescence - ENO3 Rabbit pAb (A16371)

Immunofluorescence analysis of HeLa cells using ENO3 antibody (A16371) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ENO3 Rabbit pAb (A16371)}

Immunofluorescence - ENO3 Rabbit pAb (A16371)

Immunofluorescence analysis of NIH/3T3 cells using ENO3 antibody (A16371) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ENO3 Rabbit pAb (A16371)}

Immunofluorescence - ENO3 Rabbit pAb (A16371)

Immunofluorescence analysis of PC-12 cells using ENO3 antibody (A16371) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (2)

Secondary Antibodies (26)