제품 > 항체 > 다중클론항체(pAb)

ENTPD4 Rabbit pAb (A16469)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ENTPD4 Rabbit pAb (A16469)

Western blot analysis of various lysates using ENTPD4 Rabbit pAb (A16469) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameENTPD4 Rabbit pAb
Catalog No.A16469
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the apyrase protein family. Apyrases are enzymes that catalyze the hydrolysis of nucleotide diphosphates and triphosphates in a calcium or magnesium-dependent manner. The encoded protein is an endo-apyrase and may play a role in salvaging nucleotides from lysosomes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and these isoforms may differ in divalent cation dependence and substrate specificity.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 350-470 of human ENTPD4 (NP_001122402.1).
SequenceLTPDMPYLDPCLPLDIKDEIQQNGQTIYLRGTGDFDLCRETIQPFMNKTNETQTSLNGVYQPPIHFQNSEFYGFSEFYYCTEDVLRMGGDYNAAKFTKAAKDYCATKWSILRERFDRGLYA
Gene ID
Swiss Prot
SynonymsLAP70; LALP70; LYSAL1; UDPase; NTPDase-4; ENTPD4
Calculated MW70kDa
Observed MW80kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesJurkat, Mouse heart, Rat testis
Cellular locationCytoplasmic vesicle, Golgi apparatus membrane, Multi-pass membrane protein, autophagosome membrane
    ABclonal:Western blot - ENTPD4 Rabbit pAb (A16469)}

    Western blot - ENTPD4 Rabbit pAb (A16469)

    Western blot analysis of various lysates using ENTPD4 Rabbit pAb (A16469) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.