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ERAP1 Rabbit mAb (A5978)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ERAP1 Rabbit mAb (A5978)

Western blot analysis of various lysates using ERAP1 Rabbit mAb (A5978) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - ERAP1 Rabbit mAb (A5978)

Western blot analysis of lysates from Rat liver, using ERAP1 Rabbit mAb (A5978) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - ERAP1 Rabbit mAb (A5978)

Immunohistochemistry analysis of paraffin-embedded Rat lung using ERAP1 Rabbit mAb (A5978) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ERAP1 Rabbit mAb (A5978)

Immunohistochemistry analysis of paraffin-embedded Human placenta using ERAP1 Rabbit mAb (A5978) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Overview

Product nameERAP1 Rabbit mAb
Catalog No.A5978
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2120
The protein encoded by this gene is an aminopeptidase involved in trimming HLA class I-binding precursors so that they can be presented on MHC class I molecules. The encoded protein acts as a monomer or as a heterodimer with ERAP2. This protein may also be involved in blood pressure regulation by inactivation of angiotensin II. Three transcript variants encoding two different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 842-941 of human ERAP1 (Q9NZ08).
SequenceNPVGYPLAWQFLRKNWNKLVQKFELGSSSIAHMVMGTTNQFSTRTRLEEVKGFFSSLKENGSQLRCVQQTIETIEENIGWMDKNFDKIRVWLQSEKLERM
Gene ID
Swiss Prot
SynonymsALAP; A-LAP; ARTS1; ERAAP; APPILS; ARTS-1; ERAAP1; PILSAP; PILS-AP; ERAP1
Calculated MW107kDa
Observed MW108kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHepG2, Jurkat, Mouse lung, Mouse liver, Rat liver
Cellular locationcytoplasm, cytosol, endoplasmic reticulum, endoplasmic reticulum lumen, extracellular exosome, extracellular region, extracellular space, plasma membrane

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ABclonal:Western blot - ERAP1 Rabbit mAb (A5978)}

Western blot - ERAP1 Rabbit mAb (A5978)

Western blot analysis of various lysates using ERAP1 Rabbit mAb (A5978) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - ERAP1 Rabbit mAb (A5978)}

Western blot - ERAP1 Rabbit mAb (A5978)

Western blot analysis of lysates from Rat liver, using ERAP1 Rabbit mAb (A5978) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - ERAP1 Rabbit mAb (A5978)}

Immunohistochemistry - ERAP1 Rabbit mAb (A5978)

Immunohistochemistry analysis of paraffin-embedded Rat lung using ERAP1 Rabbit mAb (A5978) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - ERAP1 Rabbit mAb (A5978)}

Immunohistochemistry - ERAP1 Rabbit mAb (A5978)

Immunohistochemistry analysis of paraffin-embedded Human placenta using ERAP1 Rabbit mAb (A5978) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)