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ESE1/ELF3 Rabbit pAb (A13489)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - ESE1/ELF3 Rabbit pAb (A13489)

Western blot analysis of various lysates, using ESE1/ELF3 Rabbit pAb (A13489) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

ABclonal:Immunoprecipitation - ESE1/ELF3 Rabbit pAb (A13489)

Immunoprecipitation analysis of 150 μg extracts of A549 cells using 3 μg ESE1/ESE1/ELF3 antibody (A13489). Western blot was performed from the immunoprecipitate using ESE1/ESE1/ELF3 antibody (A13489) at a dilution of 1:500.____Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.

Overview

Product nameESE1/ELF3 Rabbit pAb
Catalog No.A13489
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables DNA-binding transcription activator activity, RNA polymerase II-specific and sequence-specific double-stranded DNA binding activity. Involved in inflammatory response; negative regulation of transcription, DNA-templated; and positive regulation of transcription by RNA polymerase II. Located in Golgi apparatus; cytosol; and nucleoplasm.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ESE1/ESE1/ELF3 (NP_004424.3).
SequenceMAATCEISNIFSNYFSAMYSSEDSTLASVPPAATFGADDLVLTLSNPQMSLEGTEKASWLGEQPQFWSKTQVLDWISYQVEKNKYDASAIDFSRCDMDGATLCNCALEELRLVFGPLGDQLHAQLRDLTSSSSDELSWIIELLEKDGMAFQEALDPGPFDQGSPFAQELLDDGQQASPYHPGSCGAGAPSPGSSDVSTAGTGASRSSHSSDSGGSDVDLDPTDGKLFPSDGFRDCKKGDPKHGKRKRGRPRKLSKEYWDCLEGKKSKHAPRGTHLWEFIR
Gene ID
Swiss Prot
SynonymsERT; ESX; EPR-1; ESE-1; ESE1/ELF3
Calculated MW41kDa
Observed MW47kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunoprecipitation    
Positive samplesA-431, SK-BR-3
Cellular locationCytoplasm, Nucleus
Customer validation

ChIP(Homo sapiens, Mus musculus)

WB(Arabidopsis thaliana)

Documents

Certificate of Compliance

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ABclonal:Western blot - ESE1/ELF3 Rabbit pAb (A13489)}

Western blot - ESE1/ELF3 Rabbit pAb (A13489)

Western blot analysis of various lysates, using ESE1/ELF3 Rabbit pAb (A13489) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.
ABclonal:Immunoprecipitation - ESE1/ELF3 Rabbit pAb (A13489)}

Immunoprecipitation - ESE1/ELF3 Rabbit pAb (A13489)

Immunoprecipitation analysis of 150 μg extracts of A549 cells using 3 μg ESE1/ESE1/ELF3 antibody (A13489). Western blot was performed from the immunoprecipitate using ESE1/ESE1/ELF3 antibody (A13489) at a dilution of 1:500.____Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.

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