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ETS2 Rabbit pAb (A7329)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ETS2 Rabbit pAb (A7329)

Western blot analysis of extracts of various cell lines, using SPRY3 antibody (A7329) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - ETS2 Rabbit pAb (A7329)

Immunofluorescence analysis of U-2 OS cells using ETS2 antibody (A7329) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameETS2 Rabbit pAb
Catalog No.A7329
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a transcription factor which regulates genes involved in development and apoptosis. The encoded protein is also a protooncogene and shown to be involved in regulation of telomerase. A pseudogene of this gene is located on the X chromosome. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 76-170 of human ETS2 (NP_005230.1).
SequenceKAVMSQALKATFSGFKKEQRRLGIPKNPWLWSEQQVCQWLLWATNEFSLVNVNLQRFGMNGQMLCNLGKERFLELAPDFVGDILWEHLEQMIKEN
Gene ID
Swiss Prot
SynonymsETS2IT1
Calculated MW53kDa
Observed MW53kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesA-431, mouse lung
Cellular locationNucleus

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ABclonal:Western blot - ETS2 Rabbit pAb (A7329)}

Western blot - ETS2 Rabbit pAb (A7329)

Western blot analysis of extracts of various cell lines, using SPRY3 antibody (A7329) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - ETS2 Rabbit pAb (A7329)}

Immunofluorescence - ETS2 Rabbit pAb (A7329)

Immunofluorescence analysis of U-2 OS cells using ETS2 antibody (A7329) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)