제품 > 항체 > 다중클론항체(pAb)

Egr4 Rabbit pAb (A16369)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Western blot analysis of various lysates using Egr4 Rabbit pAb (A16369) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product name	Egr4 Rabbit pAb
Catalog No.	A16369
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Predicted to enable DNA-binding transcription activator activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in positive regulation of transcription by RNA polymerase II. Predicted to be located in nucleus. Is expressed in several structures, including central nervous system; genitourinary system; and tooth. Orthologous to human EGR4 (early growth response 4).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-367 of mouse Egr4 (NP_065621.1).
Sequence	MLHLSDFSGSDALLSKPTEGCAHASPELPRLPARDAPSAAAYPGGDFLSWALSSCGAGGDLTDSCFLEGPAPTPPSGLSYSGSFFIQAVPEHPHDPEALFNLMSGILGLAPFPGPEAAASRSPLDVPFPAGPDALLPDLYSPDLSSAAFPEAFWEAAPSAGAPSQCLFEPQLSPPDVKPGLRAPPASPALDAAASAFKGPYAPWELLSAGVPGNCGSQGSFQTTPEARFSAVGTKVEDLLSISCPAELPGPAARLYQAGAYDTFSLAPGDLGEGTEGLPALLTPPGGEGGSGGEGGEFLAAPPAQLSPLGLRGAATADFSKPLVADLPGGSGVAAPSSPAASFPAAKARRKGRRGGKCSARCFCPRP
Gene ID
Swiss Prot
Synonyms	NGFIC; NGF1-C; NGFI-C; pAT133; Egr4
Calculated MW	62kDa
Observed MW	58kDa

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Mouse brain, Mouse kidney
Cellular location	Nucleus