제품 > 항체 > 다중클론항체(pAb)

Epn2 Rabbit pAb (A16623)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Epn2 Rabbit pAb (A16623)

Western blot analysis of lysates from HepG2 cells, using Epn2 Rabbit pAb (A16623) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameEpn2 Rabbit pAb
Catalog No.A16623
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable clathrin binding activity and phospholipid binding activity. Acts upstream of or within Notch signaling pathway; embryonic organ development; and in utero embryonic development. Predicted to be located in intracellular membrane-bounded organelle. Predicted to be part of clathrin coat of endocytic vesicle. Predicted to be active in endosome and plasma membrane. Orthologous to human EPN2 (epsin 2).
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 100-200 of mouse Epn2 (XP_006532226.1).
SequenceIFAIQTLKDFQYIDRDGKDQGINVREKSKQLVALLKDEERLKVERVQALKTKERMAQVATGVGSNQITFGRGSSQPNLSTSYSEQEYGKAGGSPASYHGSP
Gene ID
Swiss Prot
SynonymsIbp2; 9530051D10Rik; Epn2
Calculated MW68kDa
Observed MW68kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHepG2
Cellular locationcytosol, endosome, plasma membrane

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    ABclonal:Western blot - Epn2 Rabbit pAb (A16623)}

    Western blot - Epn2 Rabbit pAb (A16623)

    Western blot analysis of lysates from HepG2 cells, using Epn2 Rabbit pAb (A16623) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)