제품 > 항체 > 단일클론항체(mAb)

F4/80 Rabbit mAb (A23788)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Immunohistochemistry analysis of paraffin-embedded mouse colon using F4/80 Rabbit mAb (A23788) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse liver using F4/80 Rabbit mAb (A23788) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse spleen using F4/80 Rabbit mAb (A23788) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product name	F4/80 Rabbit mAb
Catalog No.	A23788
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC61555

Background

Predicted to enable G protein-coupled receptor activity. Predicted to be involved in adenylate cyclase-activating G protein-coupled receptor signaling pathway. Predicted to act upstream of or within G protein-coupled receptor signaling pathway and adaptive immune response. Located in external side of plasma membrane. Is expressed in several structures, including cardiovascular system; central nervous system; genitourinary system; hemolymphoid system; and intestine. Orthologous to human ADGRE1 (adhesion G protein-coupled receptor E1).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 367-645 of mouse F4/80(NP_034260.1).
Sequence	KRILFKCKEDLILQSEQIQQCQAVQGRDLGYASFCTLVNATFTILDNTCENKSAPVSLQSAATSVSLVLEQATTWFELSKEETSTLGTILLETVESTMLAALLIPSGNASQMIQTEYLDIESKVINEECKENESINLAARGDKMNVGCFIIKESVSTGAPGVAFVSFAHMESVLNERFFEDGQSFRKLRMNSRVVGGTVTGEKKEDFSKPIIYTLQHIQPKQKSERPICVSWNTDVEDGRWTPSGCEIVEASETHTVCSCNRMANLAIIMASGELTMEF
Gene ID
Swiss Prot
Synonyms	Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Calculated MW	102KDa
Observed MW	65-250kDa

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	IHC-P 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Immunohistochemistry
Positive samples	Mouse spleen, Rat spleen
Cellular location	Cell membrane, Multi-pass membrane protein
Customer validation	IHC(Mus musculus, Homo sapiens) WB(Rattus norvegicus) IF(Mus musculus) WB(Mus musculus)