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FABP1 Rabbit pAb (A24759)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - FABP1 Rabbit pAb (A24759)

Western blot analysis of various lysates using FABP1 Rabbit pAb (A24759) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): Mouse brain.
Exposure time: 90s.

ABclonal:Immunofluorescence - FABP1 Rabbit pAb (A24759)

Immunofluorescence analysis of Hep G2 cells using FABP1 Rabbit pAb (A24759) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - FABP1 Rabbit pAb (A24759)

Immunofluorescence analysis of U-2 OS cells using FABP1 Rabbit pAb (A24759) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameFABP1 Rabbit pAb
Catalog No.A24759
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the fatty acid binding protein found in liver. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. This protein and FABP6 (the ileal fatty acid binding protein) are also able to bind bile acids. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human FABP1(NP_001434.1).
SequenceMSFSGKYQLQSQENFEAFMKAIGLPEELIQKGKDIKGVSEIVQNGKHFKFTITAGSKVIQNEFTVGEECELETMTGEKVKTVVQLEGDNKLVTTFKNIKS
Gene ID
Swiss Prot
SynonymsFABP1; FABPL; L-FABP; fatty acid binding protein 1
Calculated MW14kDa
Observed MW14kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse liver, Rat liver
Cellular locationCytoplasm

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - FABP1 Rabbit pAb (A24759)}

    Western blot - FABP1 Rabbit pAb (A24759)

    Western blot analysis of various lysates using FABP1 Rabbit pAb (A24759) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Negative control (NC): Mouse brain.
    Exposure time: 90s.
    ABclonal:Immunofluorescence - FABP1 Rabbit pAb (A24759)}

    Immunofluorescence - FABP1 Rabbit pAb (A24759)

    Immunofluorescence analysis of Hep G2 cells using FABP1 Rabbit pAb (A24759) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - FABP1 Rabbit pAb (A24759)}

    Immunofluorescence - FABP1 Rabbit pAb (A24759)

    Immunofluorescence analysis of U-2 OS cells using FABP1 Rabbit pAb (A24759) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)