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FABP4 Rabbit mAb (A11481)

Publications (9) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - FABP4 Rabbit mAb (A11481)

Western blot analysis of various lysates using FABP4 Rabbit mAb (A11481) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameFABP4 Rabbit mAb
Catalog No.A11481
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0616
FABP4 encodes the fatty acid binding protein found in adipocytes. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human FABP4 (P15090).
SequenceMCDAFVGTWKLVSSENFDDYMKEVGVGFATRKVAGMAKPNMIISVNGDVITIKSESTFKNTEISFILGQEFDEVTADDRKVKSTITLDGGVLVHVQKWDG
Gene ID
Swiss Prot
SynonymsaP2; ALBP; AFABP; A-FABP; HEL-S-104; FABP4
Calculated MW15kDa
Observed MW15kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:4000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse heart, Mouse thymus, Rat heart, Rat thymus
Cellular locationCytoplasm, Nucleus.
Customer validation

WB(Sus scrofa, Mus musculus, Oryctolagus cuniculus, Rattus norvegicus)

Documents

Certificate of Compliance

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ABclonal:Western blot - FABP4 Rabbit mAb (A11481)}

Western blot - FABP4 Rabbit mAb (A11481)

Western blot analysis of various lysates using FABP4 Rabbit mAb (A11481) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A11481? Please let us know so that we can cite the reference in this datasheet.

항체 (3)

ELISA 키트 (1)

재조합 단백질 (2)

Secondary Antibodies (26)