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FAIM3 Rabbit pAb (A6320)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - FAIM3 Rabbit pAb (A6320)

Western blot analysis of extracts of various cell lines, using FAIM3 antibody (A6320) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

Overview

Product nameFAIM3 Rabbit pAb
Catalog No.A6320
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Fc receptors specifically bind to the Fc region of immunoglobulins (Igs) to mediate the unique functions of each Ig class. FAIM3 encodes an Fc receptor for IgM (see MIM 147020) (Kubagawa et al., 2009 [PubMed 19858324]; Shima et al., 2010 [PubMed 20042454]).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 18-251 of human FAIM3 (NP_005440.1).
SequenceRILPEVKVEGELGGSVTIKCPLPEMHVRIYLCREMAGSGTCGTVVSTTNFIKAEYKGRVTLKQYPRKNLFLVEVTQLTESDSGVYACGAGMNTDRGKTQKVTLNVHSEYEPSWEEQPMPETPKWFHLPYLFQMPAYASSSKFVTRVTTPAQRGKVPPVHHSSPTTQITHRPRVSRASSVAGDKPRTFLPSTTASKISALEGLLKPQTPSYNHHTRLHRQRALDYGSQSGREGQG
Gene ID
Swiss Prot
SynonymsTOSO; FAIM3; FcmuR
Calculated MW43kDa
Observed MW40kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesJurkat, U-251MG, SW480
Cellular locationMembrane, Secreted, Single-pass membrane protein
Customer validation

WB(Rattus norvegicus)

Documents

Certificate of Compliance

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    ABclonal:Western blot - FAIM3 Rabbit pAb (A6320)}

    Western blot - FAIM3 Rabbit pAb (A6320)

    Western blot analysis of extracts of various cell lines, using FAIM3 antibody (A6320) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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