제품 > 항체 > 단일클론항체(mAb)

FATP4/SLC27A4 Rabbit mAb (A21969)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - FATP4/SLC27A4 Rabbit mAb (A21969)

Western blot analysis of extracts of Mouse brain, using FATP4/SLC27A4 antibody (A21969) at1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - FATP4/SLC27A4 Rabbit mAb (A21969)

Western blot analysis of extracts of HepG2 cells, using FATP4/SLC27A4 antibody (A21969) at1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

Overview

Product nameFATP4/SLC27A4 Rabbit mAb
Catalog No.A21969
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC53579
This gene encodes a member of a family of fatty acid transport proteins, which are involved in translocation of long-chain fatty acids cross the plasma membrane. This protein is expressed at high levels on the apical side of mature enterocytes in the small intestine, and appears to be the principal fatty acid transporter in enterocytes. Clinical studies suggest this gene as a candidate gene for the insulin resistance syndrome. Mutations in this gene have been associated with ichthyosis prematurity syndrome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 43-237 of human FATP4/SLC27A4 (NP_005085.2).
SequenceRVFIKTIRRDIFGGLVLLKVKAKVRQCLQERRTVPILFASTVRRHPDKTALIFEGTDTHWTFRQLDEYSSSVANFLQARGLASGDVAAIFMENRNEFVGLWLGMAKLGVEAALINTNLRRDALLHCLTTSRARALVFGSEMASAICEVHASLDPSLSLFCSGSWEPGAVPPSTEHLDPLLKDAPKHLPSCPDKGF
Gene ID
Swiss Prot
SynonymsIPS; FATP4; ACSVL4
Calculated MW72kDa
Observed MW65kDa/70kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHepG2, Mouse brain
Cellular locationEndoplasmic reticulum membrane, Membrane, Multi-pass membrane protein

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - FATP4/SLC27A4 Rabbit mAb (A21969)}

Western blot - FATP4/SLC27A4 Rabbit mAb (A21969)

Western blot analysis of extracts of Mouse brain, using FATP4/SLC27A4 antibody (A21969) at1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - FATP4/SLC27A4 Rabbit mAb (A21969)}

Western blot - FATP4/SLC27A4 Rabbit mAb (A21969)

Western blot analysis of extracts of HepG2 cells, using FATP4/SLC27A4 antibody (A21969) at1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (1)

Secondary Antibodies (26)