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FBP1 Rabbit mAb (A11664)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - FBP1 Rabbit mAb (A11664)

Western blot analysis of lysates from MCF7 cells, using FBP1 Rabbit mAb (A11664) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunofluorescence - FBP1 Rabbit mAb (A11664)

Immunofluorescence analysis of HeLa cells using FBP1 Rabbit mAb (A11664) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - FBP1 Rabbit mAb (A11664)

Immunofluorescence analysis of paraffin-embedded rat kidney using FBP1 Rabbit mAb (A11664) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - FBP1 Rabbit mAb (A11664)

Immunofluorescence analysis of paraffin-embedded mouse kidney using FBP1 Rabbit mAb (A11664) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameFBP1 Rabbit mAb
Catalog No.A11664
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0664
Fructose-1, 6-bisphosphatase 1, a gluconeogenesis regulatory enzyme, catalyzes the hydrolysis of fructose 1, 6-bisphosphate to fructose 6-phosphate and inorganic phosphate. Fructose-1, 6-diphosphatase deficiency is associated with hypoglycemia and metabolic acidosis.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 239-338 of human FBP1 (P09467).
SequenceAPYGARYVGSMVADVHRTLVYGGIFLYPANKKSPNGKLRLLYECNPMAYVMEKAGGMATTGKEAVLDVIPTDIHQRAPVILGSPDDVLEFLKVYEKHSAQ
Gene ID
Swiss Prot
SynonymsFBP; FBP1
Calculated MW37kDa
Observed MW40kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:6000
  • IF/ICC 1:100 - 1:400
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMCF7
Cellular locationcytoplasm, cytosol, extracellular exosome, nucleus.
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - FBP1 Rabbit mAb (A11664)}

Western blot - FBP1 Rabbit mAb (A11664)

Western blot analysis of lysates from MCF7 cells, using FBP1 Rabbit mAb (A11664) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunofluorescence - FBP1 Rabbit mAb (A11664)}

Immunofluorescence - FBP1 Rabbit mAb (A11664)

Immunofluorescence analysis of HeLa cells using FBP1 Rabbit mAb (A11664) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - FBP1 Rabbit mAb (A11664)}

Immunofluorescence - FBP1 Rabbit mAb (A11664)

Immunofluorescence analysis of paraffin-embedded rat kidney using FBP1 Rabbit mAb (A11664) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - FBP1 Rabbit mAb (A11664)}

Immunofluorescence - FBP1 Rabbit mAb (A11664)

Immunofluorescence analysis of paraffin-embedded mouse kidney using FBP1 Rabbit mAb (A11664) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

ELISA 키트 (1)

Secondary Antibodies (26)