제품 > 항체 > 다중클론항체(pAb)

FEM1B Rabbit pAb (A12802)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - FEM1B Rabbit pAb (A12802)

Western blot analysis of lysates from A-549 cells, using FEM1B Rabbit pAb (A12802) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

Overview

Product nameFEM1B Rabbit pAb
Catalog No.A12802
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an ankyrin repeat protein that belongs to the death receptor-associated family of proteins and plays a role in mediating apoptosis. The encoded protein is also thought to function in the replication stress-induced checkpoint signaling pathway via interaction with checkpoint kinase 1.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 368-627 of human FEM1B (NP_056137.1).
SequenceLHALHLRQKGNRNTHKDLLRFAQVFSQMIHLNETVKAPDIECVLRCSVLEIEQSMNRVKNISDADVHNAMDNYECNLYTFLYLVCISTKTQCSEEDQCKINKQIYNLIHLDPRTREGFTLLHLAVNSNTPVDDFHTNDVCSFPNALVTKLLLDCGAEVNAVDNEGNSALHIIVQYNRPISDFLTLHSIIISLVEAGAHTDMTNKQNKTPLDKSTTGVSEILLKTQMKMSLKCLAARAVRANDINYQDQIPRTLEEFVGFH
Gene ID
Swiss Prot
SynonymsF1AA; F1A-ALPHA; FEM1-beta; FEM1B
Calculated MW70kDa
Observed MW75kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesA-549
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Homo sapiens)

    ABclonal:Western blot - FEM1B Rabbit pAb (A12802)}

    Western blot - FEM1B Rabbit pAb (A12802)

    Western blot analysis of lysates from A-549 cells, using FEM1B Rabbit pAb (A12802) at 1:3000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A12802? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)