제품 > 항체 > 다중클론항체(pAb)

FEZ1 Rabbit pAb (A15362)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - FEZ1 Rabbit pAb (A15362)

Western blot analysis of various lysates using FEZ1 Rabbit pAb (A15362) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 10s.

Overview

Product nameFEZ1 Rabbit pAb
Catalog No.A15362
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is an ortholog of the C. elegans unc-76 gene, which is necessary for normal axonal bundling and elongation within axon bundles. Expression of this gene in C. elegans unc-76 mutants can restore to the mutants partial locomotion and axonal fasciculation, suggesting that it also functions in axonal outgrowth. The N-terminal half of the gene product is highly acidic. Alternatively spliced transcript variants encoding different isoforms of this protein have been described.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human FEZ1 (NP_005094.1).
SequenceMEAPLVSLDEEFEDLRPSCSEDPEEKPQCFYGSSPHHLEDPSLSELENFSSEIISFKSMEDLVNEFDEKLNVCFRNYNAKTENLAPVKNQLQIQEEEETLQDEEVWDALTDNYIPSLSEDWRDPNIEALNGNCSDTEIHEKEEEEFNEKSENDSGINEEP
Gene ID
Swiss Prot
SynonymsUNC-76; FEZ1
Calculated MW45kDa
Observed MW62kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse brain, Rat brain
Cellular locationCell membrane, Cytoplasm, centrosome, cytoskeleton, microtubule organizing center
Customer validation

WB(Mus musculus)

IHC(Mus musculus)

ABclonal:Western blot - FEZ1 Rabbit pAb (A15362)}

Western blot - FEZ1 Rabbit pAb (A15362)

Western blot analysis of various lysates using FEZ1 Rabbit pAb (A15362) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 10s.

* For research use only. Not for therapeutic or diagnostic purposes.

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