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FOXG1 Rabbit pAb (A16851)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - FOXG1 Rabbit pAb (A16851)

Western blot analysis of various lysates using FOXG1 Rabbit pAb (A16851) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - FOXG1 Rabbit pAb (A16851)

Immunofluorescence analysis of U2OS cells using FOXG1 Rabbit pAb (A16851) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameFOXG1 Rabbit pAb
Catalog No.A16851
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This locus encodes a member of the fork-head transcription factor family. The encoded protein, which functions as a transcriptional repressor, is highly expressed in neural tissues during brain development. Mutations at this locus have been associated with Rett syndrome and a diverse spectrum of neurodevelopmental disorders defined as part of the FOXG1 syndrome. This gene is disregulated in many types of cancer and is the target of multiple microRNAs that regulate the proliferation of tumor cells.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 400-489 of human FOXG1 (NP_005240.3).
SequenceYSLNPCSVNLLAGQTSYFFPHVPHPSMTSQSSTSMSARAASSSTSPQAPSTLPCESLRPSLPSFTTGLSGGLSDYFTHQNQGSSSNPLIH
Gene ID
Swiss Prot
SynonymsBF1; BF2; QIN; FKH2; HBF2; HFK1; HFK2; HFK3; KHL2; FHKL3; FKHL1; FKHL2; FKHL3; FKHL4; HBF-1; HBF-2; HBF-3; FOXG1A; FOXG1B; FOXG1C; HBF-G2; FOXG1
Calculated MW52kDa
Observed MW52kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:100
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples293T, SH-SY5Y, Mouse brain, Mouse spleen, Rat brain
Cellular locationNucleus.
Customer validation

WB(Rattus norvegicus)

Documents

Certificate of Compliance

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ABclonal:Western blot - FOXG1 Rabbit pAb (A16851)}

Western blot - FOXG1 Rabbit pAb (A16851)

Western blot analysis of various lysates using FOXG1 Rabbit pAb (A16851) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - FOXG1 Rabbit pAb (A16851)}

Immunofluorescence - FOXG1 Rabbit pAb (A16851)

Immunofluorescence analysis of U2OS cells using FOXG1 Rabbit pAb (A16851) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A16851? Please let us know so that we can cite the reference in this datasheet.

Secondary Antibodies (26)