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FOXO3A Rabbit pAb (A0102)

Publications (28) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - FOXO3A Rabbit pAb (A0102)

Western blot analysis of various lysates using FOXO3A Rabbit pAb (A0102) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - FOXO3A Rabbit pAb (A0102)

Immunohistochemistry analysis of paraffin-embedded Human colon using FOXO3A Rabbit pAb (A0102) at dilution of 1:50 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - FOXO3A Rabbit pAb (A0102)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using FOXO3A Rabbit pAb (A0102) at dilution of 1:50 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - FOXO3A Rabbit pAb (A0102)

Immunofluorescence analysis of C6 cells using FOXO3A Rabbit pAb (A0102) at dilution of 100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - FOXO3A Rabbit pAb (A0102)

Immunofluorescence analysis of NIH-3T3 cells using FOXO3A Rabbit pAb (A0102) at dilution of 100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameFOXO3A Rabbit pAb
Catalog No.A0102
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables DNA binding activity; DNA-binding transcription factor activity, RNA polymerase II-specific; and mitochondrial transcription factor activity. Involved in several processes, including mitochondrial transcription; positive regulation of muscle atrophy; and positive regulation of pri-miRNA transcription by RNA polymerase II. Acts upstream of or within several processes, including extrinsic apoptotic signaling pathway in absence of ligand; female gonad development; and neuronal stem cell population maintenance. Located in cytosol; mitochondrial outer membrane; and nucleus. Part of protein-containing complex. Colocalizes with mitochondrial matrix. Is expressed in several structures, including alimentary system; brain; early embryo; genitourinary system; and hemolymphoid system. Used to study dermoid cyst of ovary. Orthologous to human FOXO3 (forkhead box O3).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 558-635 of mouse FOXO3A (NP_062714.1).
SequenceLSDSSSLGSAKHQQQSPASQSMQTLSDSLSGSSLYSASANLPVMGHDKFPSDLDLDMFNGSLECDMESIIRSELMDAD
Gene ID
Swiss Prot
SynonymsFkhr2; FKHRL1; Foxo3a; 1110048B16Rik; 2010203A17Rik; FOXO3A
Calculated MW71kDa
Observed MW80-90kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, Jurkat, 293T, PC-3
Cellular locationCytoplasm, Nucleus, cytosol.
Customer validation

WB(Danio rerio, Mus musculus, Homo sapiens, Rattus norvegicus, Mus musculus)

Co-IP(Rattus norvegicus)

IP(Rattus norvegicus)

IF(Mus musculus)

IP(Mus musculus)

Documents

Certificate of Compliance

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    ABclonal:Western blot - FOXO3A Rabbit pAb (A0102)}

    Western blot - FOXO3A Rabbit pAb (A0102)

    Western blot analysis of various lysates using FOXO3A Rabbit pAb (A0102) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.
    ABclonal:Immunohistochemistry - FOXO3A Rabbit pAb (A0102)}

    Immunohistochemistry - FOXO3A Rabbit pAb (A0102)

    Immunohistochemistry analysis of paraffin-embedded Human colon using FOXO3A Rabbit pAb (A0102) at dilution of 1:50 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - FOXO3A Rabbit pAb (A0102)}

    Immunohistochemistry - FOXO3A Rabbit pAb (A0102)

    Immunohistochemistry analysis of paraffin-embedded Mouse kidney using FOXO3A Rabbit pAb (A0102) at dilution of 1:50 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunofluorescence - FOXO3A Rabbit pAb (A0102)}

    Immunofluorescence - FOXO3A Rabbit pAb (A0102)

    Immunofluorescence analysis of C6 cells using FOXO3A Rabbit pAb (A0102) at dilution of 100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - FOXO3A Rabbit pAb (A0102)}

    Immunofluorescence - FOXO3A Rabbit pAb (A0102)

    Immunofluorescence analysis of NIH-3T3 cells using FOXO3A Rabbit pAb (A0102) at dilution of 100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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