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FOXP1 Rabbit mAb (A23442)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - FOXP1 Rabbit mAb (A23442)

Western blot analysis of various lysates, using FOXP1 Rabbit mAb (A23442) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - FOXP1 Rabbit mAb (A23442)

Immunohistochemistry analysis of paraffin-embedded Human colon using FOXP1 Rabbit mAb (A23442) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - FOXP1 Rabbit mAb (A23442)

Immunohistochemistry analysis of paraffin-embedded Mouse intestin using FOXP1 Rabbit mAb (A23442) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - FOXP1 Rabbit mAb (A23442)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using FOXP1 Rabbit mAb (A23442) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - FOXP1 Rabbit mAb (A23442)

Immunohistochemistry analysis of paraffin-embedded Mouse intestin using FOXP1 Rabbit mAb (A23442) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - FOXP1 Rabbit mAb (A23442)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using FOXP1 Rabbit mAb (A23442) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - FOXP1 Rabbit mAb (A23442)

Immunohistochemistry analysis of paraffin-embedded Human liver using FOXP1 Rabbit mAb (A23442) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - FOXP1 Rabbit mAb (A23442)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using FOXP1 Rabbit mAb (A23442) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - FOXP1 Rabbit mAb (A23442)

Immunohistochemistry analysis of paraffin-embedded Mouse liver using FOXP1 Rabbit mAb (A23442) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - FOXP1 Rabbit mAb (A23442)

Immunohistochemistry analysis of paraffin-embedded Rat brain using FOXP1 Rabbit mAb (A23442) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - FOXP1 Rabbit mAb (A23442)

Confocal imaging of NIH-3T3 cells using FOXP1 Rabbit mAb (A9540, dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

Overview

Product nameFOXP1 Rabbit mAb
Catalog No.A23442
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC57682
This gene belongs to subfamily P of the forkhead box (FOX) transcription factor family. Forkhead box transcription factors play important roles in the regulation of tissue- and cell type-specific gene transcription during both development and adulthood. Forkhead box P1 protein contains both DNA-binding- and protein-protein binding-domains. This gene may act as a tumor suppressor as it is lost in several tumor types and maps to a chromosomal region (3p14.1) reported to contain a tumor suppressor gene(s). Alternative splicing results in multiple transcript variants encoding different isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 550-677 of human FOXP1. (NP_116071.2).
SequenceSGNPSLIKNMQSSHAYCTPLNAALQASMAENSIPLYTTASMGNPTLGNLASAIREELNGAMEHTNSNESDSSPGRSPMQAVHPVHVKEEPLDPEEAEGPLSLVTTANHSPDFDHDRDYEDEPVNEDME
Gene ID
Swiss Prot
SynonymsMFH; QRF1; 12CC4; hFKH1B; HSPC215; FOXP1
Calculated MW75kDa
Observed MW70-90kd
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:100 - 1:500
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesA549, Mouse embryo, Rat brain
Cellular locationNucleus

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - FOXP1 Rabbit mAb (A23442)}

    Western blot - FOXP1 Rabbit mAb (A23442)

    Western blot analysis of various lysates, using FOXP1 Rabbit mAb (A23442) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Immunohistochemistry - FOXP1 Rabbit mAb (A23442)}

    Immunohistochemistry - FOXP1 Rabbit mAb (A23442)

    Immunohistochemistry analysis of paraffin-embedded Human colon using FOXP1 Rabbit mAb (A23442) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - FOXP1 Rabbit mAb (A23442)}

    Immunohistochemistry - FOXP1 Rabbit mAb (A23442)

    Immunohistochemistry analysis of paraffin-embedded Mouse intestin using FOXP1 Rabbit mAb (A23442) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - FOXP1 Rabbit mAb (A23442)}

    Immunohistochemistry - FOXP1 Rabbit mAb (A23442)

    Immunohistochemistry analysis of paraffin-embedded Rat spleen using FOXP1 Rabbit mAb (A23442) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - FOXP1 Rabbit mAb (A23442)}

    Immunohistochemistry - FOXP1 Rabbit mAb (A23442)

    Immunohistochemistry analysis of paraffin-embedded Mouse intestin using FOXP1 Rabbit mAb (A23442) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - FOXP1 Rabbit mAb (A23442)}

    Immunohistochemistry - FOXP1 Rabbit mAb (A23442)

    Immunohistochemistry analysis of paraffin-embedded Rat spleen using FOXP1 Rabbit mAb (A23442) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - FOXP1 Rabbit mAb (A23442)}

    Immunohistochemistry - FOXP1 Rabbit mAb (A23442)

    Immunohistochemistry analysis of paraffin-embedded Human liver using FOXP1 Rabbit mAb (A23442) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - FOXP1 Rabbit mAb (A23442)}

    Immunohistochemistry - FOXP1 Rabbit mAb (A23442)

    Immunohistochemistry analysis of paraffin-embedded Mouse spleen using FOXP1 Rabbit mAb (A23442) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - FOXP1 Rabbit mAb (A23442)}

    Immunohistochemistry - FOXP1 Rabbit mAb (A23442)

    Immunohistochemistry analysis of paraffin-embedded Mouse liver using FOXP1 Rabbit mAb (A23442) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - FOXP1 Rabbit mAb (A23442)}

    Immunohistochemistry - FOXP1 Rabbit mAb (A23442)

    Immunohistochemistry analysis of paraffin-embedded Rat brain using FOXP1 Rabbit mAb (A23442) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - FOXP1 Rabbit mAb (A23442)}

    Immunofluorescence - FOXP1 Rabbit mAb (A23442)

    Confocal imaging of NIH-3T3 cells using FOXP1 Rabbit mAb (A9540, dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

    * For research use only. Not for therapeutic or diagnostic purposes.

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