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Filamin A Rabbit pAb (A16376)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Filamin A Rabbit pAb (A16376)

Western blot analysis of various lysates using Filamin A Rabbit pAb (A16376) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Filamin A Rabbit pAb (A16376)

Immunohistochemistry analysis of paraffin-embedded rat heart using Filamin A Rabbit pAb (A16376) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Filamin A Rabbit pAb (A16376)

Immunohistochemistry analysis of paraffin-embedded human breast cancer using Filamin A Rabbit pAb (A16376) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Filamin A Rabbit pAb (A16376)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Filamin A Rabbit pAb (A16376) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Filamin A Rabbit pAb (A16376)

Immunofluorescence analysis of HeLa cells using Filamin A Rabbit pAb (A16376) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Filamin A Rabbit pAb (A16376)

Immunofluorescence analysis of NIH/3T3 cells using Filamin A Rabbit pAb (A16376) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Filamin A Rabbit pAb (A16376)

Immunofluorescence analysis of PC-12 cells using Filamin A Rabbit pAb (A16376) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameFilamin A Rabbit pAb
Catalog No.A16376
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. The encoded protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. This protein interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in this gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 2348-2647 of human Filamin A (NP_001104026.1).
SequenceNQPASFAVSLNGAKGAIDAKVHSPSGALEECYVTEIDQDKYAVRFIPRENGVYLIDVKFNGTHIPGSPFKIRVGEPGHGGDPGLVSAYGAGLEGGVTGNPAEFVVNTSNAGAGALSVTIDGPSKVKMDCQECPEGYRVTYTPMAPGSYLISIKYGGPYHIGGSPFKAKVTGPRLVSNHSLHETSSVFVDSLTKATCAPQHGAPGPGPADASKVVAKGLGLSKAYVGQKSSFTVDCSKAGNNMLLVGVHGPRTPCEEILVKHVGSRLYSVSYLLKDKGEYTLVVKWGDEHIPGSPYRVVVP
Gene ID
Swiss Prot
SynonymsFLN; FMD; MNS; OPD; ABPX; CSBS; CVD1; FGS2; FLN1; NHBP; OPD1; OPD2; XLVD; XMVD; FLN-A; ABP-280; Filamin A
Calculated MW281kDa
Observed MW300kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesJurkat, HeLa, NIH/3T3, 293T, U-251MG, Mouse lung
Cellular locationCytoplasm, cell cortex, cytoskeleton
Customer validation

WB(Homo sapiens)

IF(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - Filamin A Rabbit pAb (A16376)}

Western blot - Filamin A Rabbit pAb (A16376)

Western blot analysis of various lysates using Filamin A Rabbit pAb (A16376) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - Filamin A Rabbit pAb (A16376)}

Immunohistochemistry - Filamin A Rabbit pAb (A16376)

Immunohistochemistry analysis of paraffin-embedded rat heart using Filamin A Rabbit pAb (A16376) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Filamin A Rabbit pAb (A16376)}

Immunohistochemistry - Filamin A Rabbit pAb (A16376)

Immunohistochemistry analysis of paraffin-embedded human breast cancer using Filamin A Rabbit pAb (A16376) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Filamin A Rabbit pAb (A16376)}

Immunohistochemistry - Filamin A Rabbit pAb (A16376)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Filamin A Rabbit pAb (A16376) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Filamin A Rabbit pAb (A16376)}

Immunofluorescence - Filamin A Rabbit pAb (A16376)

Immunofluorescence analysis of HeLa cells using Filamin A Rabbit pAb (A16376) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Filamin A Rabbit pAb (A16376)}

Immunofluorescence - Filamin A Rabbit pAb (A16376)

Immunofluorescence analysis of NIH/3T3 cells using Filamin A Rabbit pAb (A16376) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Filamin A Rabbit pAb (A16376)}

Immunofluorescence - Filamin A Rabbit pAb (A16376)

Immunofluorescence analysis of PC-12 cells using Filamin A Rabbit pAb (A16376) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

Secondary Antibodies (26)