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Folate Binding Protein(FBP) / FOLR1 Rabbit mAb (A20726)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Folate Binding Protein(FBP) / FOLR1 Rabbit mAb (A20726)

Western blot analysis of lysates from HeLa cells, using Folate Binding Protein(FBP) / FOLR1 Rabbit mAb (A20726) at1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - Folate Binding Protein(FBP) / FOLR1 Rabbit mAb (A20726)

Western blot analysis of various lysates using Folate Binding Protein(FBP) / FOLR1 Rabbit mAb (A20726) at1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameFolate Binding Protein(FBP) / FOLR1 Rabbit mAb
Catalog No.A20726
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC50859
The protein encoded by this gene is a member of the folate receptor family. Members of this gene family bind folic acid and its reduced derivatives, and transport 5-methyltetrahydrofolate into cells. This gene product is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. Mutations in this gene have been associated with neurodegeneration due to cerebral folate transport deficiency. Due to the presence of two promoters, multiple transcription start sites, and alternative splicing, multiple transcript variants encoding the same protein have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human Folate Binding Protein(FBP) / FOLR1 (NP_000793.1).
SequenceMAQRMTTQLLLLLVWVAVVGEAQTRIAWARTELLNVCMNAKHHKEKPGPEDKLHEQCRPWRKNACCSTNTSQEAHKDVSYLYRFNWNHCGEMAPACKRHF
Gene ID
Swiss Prot
SynonymsFBP; FOLR; NCFTD; FRalpha; Folate Binding Protein(FBP) / FOLR1
Calculated MW30kDa
Observed MW38kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, Mouse kidney, Rat kidney
Cellular locationApical cell membrane, Cell membrane, Cytoplasmic vesicle, Endosome, GPI-anchor, Lipid-anchor, Secreted, clathrin-coated vesicle.
Customer validation

WB(Mus musculus, Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - Folate Binding Protein(FBP) / FOLR1 Rabbit mAb (A20726)}

Western blot - Folate Binding Protein(FBP) / FOLR1 Rabbit mAb (A20726)

Western blot analysis of lysates from HeLa cells, using Folate Binding Protein(FBP) / FOLR1 Rabbit mAb (A20726) at1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - Folate Binding Protein(FBP) / FOLR1 Rabbit mAb (A20726)}

Western blot - Folate Binding Protein(FBP) / FOLR1 Rabbit mAb (A20726)

Western blot analysis of various lysates using Folate Binding Protein(FBP) / FOLR1 Rabbit mAb (A20726) at1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A20726? Please let us know so that we can cite the reference in this datasheet.

재조합 단백질 (1)

항체 (3)

Secondary Antibodies (26)