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GABA transaminase (ABAT) Rabbit pAb (A5299)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GABA transaminase (ABAT) Rabbit pAb (A5299)

Western blot analysis of various lysates, using GABA transaminase (ABAT) Rabbit pAb (A5299) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - GABA transaminase (ABAT) Rabbit pAb (A5299)

Immunofluorescence analysis of paraffin-embedded mouse brain using GABA transaminase (GABA transaminase (ABAT)) Rabbit pAb (A5299) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameGABA transaminase (ABAT) Rabbit pAb
Catalog No.A5299
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 29-300 of human GABA transaminase (GABA transaminase (ABAT)) (NP_001120920.1).
SequenceSQAAAKVDVEFDYDGPLMKTEVPGPRSQELMKQLNIIQNAEAVHFFCNYEESRGNYLVDVDGNRMLDLYSQISSVPIGYSHPALLKLIQQPQNASMFVNRPALGILPPENFVEKLRQSLLSVAPKGMSQLITMACGSCSNENALKTIFMWYRSKERGQRGFSQEELETCMINQAPGCPDYSILSFMGAFHGRTMGCLATTHSKAIHKIDIPSFDWPIAPFPRLKYPLEEFVKENQQEEARCLEEVEDLIVKYRKKKKTVAGIIVEPIQSEGG
Gene ID
Swiss Prot
SynonymsGABAT; NPD009; GABA-AT; GABA transaminase (ABAT)
Calculated MW56kDa
Observed MW50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHepG2, Mouse kidney, Rat kidney
Cellular locationMitochondrion matrix.
Customer validation

WB(Rattus norvegicus)

Documents

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ABclonal:Western blot - GABA transaminase (ABAT) Rabbit pAb (A5299)}

Western blot - GABA transaminase (ABAT) Rabbit pAb (A5299)

Western blot analysis of various lysates, using GABA transaminase (ABAT) Rabbit pAb (A5299) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - GABA transaminase (ABAT) Rabbit pAb (A5299)}

Immunofluorescence - GABA transaminase (ABAT) Rabbit pAb (A5299)

Immunofluorescence analysis of paraffin-embedded mouse brain using GABA transaminase (GABA transaminase (ABAT)) Rabbit pAb (A5299) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

Secondary Antibodies (26)