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GALNT2 Rabbit pAb (A6910)

Reviews (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GALNT2 Rabbit pAb (A6910)

Western blot analysis of various lysates using GALNT2 Rabbit pAb (A6910) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - GALNT2 Rabbit pAb (A6910)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using GALNT2 Rabbit pAb (A6910) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - GALNT2 Rabbit pAb (A6910)

Immunofluorescence analysis of C6 cells using GALNT2 Rabbit pAb (A6910) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GALNT2 Rabbit pAb (A6910)

Immunofluorescence analysis of L929 cells using GALNT2 Rabbit pAb (A6910) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GALNT2 Rabbit pAb (A6910)

Immunofluorescence analysis of U-2 OS cells using GALNT2 Rabbit pAb (A6910) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for GALNT2 Rabbit pAb(A6910)
Review
ABclonal: review for GALNT2 Rabbit pAb(A6910)

Overview

Product nameGALNT2 Rabbit pAb
Catalog No.A6910
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the glycosyltransferase 2 protein family. Members of this family initiate mucin-type O-glycoslation of peptides in the Golgi apparatus. The encoded protein may be involved in O-linked glycosylation of the immunoglobulin A1 hinge region. This gene may influence triglyceride levels, and may be involved Type 2 diabetes, as well as several types of cancer. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 442-571 of human GALNT2 (NP_004472.1).
SequenceHQDIAFGALQQGTNCLDTLGHFADGVVGVYECHNAGGNQEWALTKEKSVKHMDLCLTVVDRAPGSLIKLQGCRENDSRQKWEQIEGNSKLRHVGSNLCLDSRTAKSGGLSVEVCGPALSQQWKFTLNLQQ
Gene ID
Swiss Prot
SynonymsCDG2T; GalNAc-T2; GALNT2
Calculated MW65kDa
Observed MW65kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:100 - 1:500
  • IF/ICC 1:50 - 1:100
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, Jurkat, HepG2, mouse lung
Cellular locationGolgi apparatus, Golgi stack membrane, Secreted, Single-pass type II membrane protein.
Customer validation

(Hela, HepG2)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - GALNT2 Rabbit pAb (A6910)}

    Western blot - GALNT2 Rabbit pAb (A6910)

    Western blot analysis of various lysates using GALNT2 Rabbit pAb (A6910) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunohistochemistry - GALNT2 Rabbit pAb (A6910)}

    Immunohistochemistry - GALNT2 Rabbit pAb (A6910)

    Immunohistochemistry analysis of paraffin-embedded Rat kidney using GALNT2 Rabbit pAb (A6910) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - GALNT2 Rabbit pAb (A6910)}

    Immunofluorescence - GALNT2 Rabbit pAb (A6910)

    Immunofluorescence analysis of C6 cells using GALNT2 Rabbit pAb (A6910) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - GALNT2 Rabbit pAb (A6910)}

    Immunofluorescence - GALNT2 Rabbit pAb (A6910)

    Immunofluorescence analysis of L929 cells using GALNT2 Rabbit pAb (A6910) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - GALNT2 Rabbit pAb (A6910)}

    Immunofluorescence - GALNT2 Rabbit pAb (A6910)

    Immunofluorescence analysis of U-2 OS cells using GALNT2 Rabbit pAb (A6910) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)