제품 > 항체 > 단일클론항체(mAb)

GATA3 Rabbit mAb (A19636)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Chromatin immunoprecipitations were performed with cross-linked chromatin from 293T cells transfected with GATA3 and GATA3 (A19636). The ChIP sequencing results indicate the enrichment pattern of GATA3 in selected genomic region and representative gene loci (SH3RF3), as shown in figure.

ABclonal:Western blot - GATA3 Rabbit mAb (A19636)

Western blot analysis of various lysates using GATA3 Rabbit mAb (A19636) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunoprecipitation - GATA3 Rabbit mAb (A19636)

Immunoprecipitation analysis of 300 μg extracts of Jurkat cells using 3 μg GATA3 antibody (A19636). Western blot was performed from the immunoprecipitate using GATA3 antibody (A19636) at a dilution of 1:500.

ABclonal:Chromatin Immunoprecipitation - GATA3 Rabbit mAb (A19636)

Chromatin immunoprecipitation analysis of extracts of MCF7 cells, using GATA3 Rabbit mAb antibody (A19636) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Overview

Product name	GATA3 Rabbit mAb
Catalog No.	A19636
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0115

Background

This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human GATA3 (P23771).
Sequence	MEVTADQPRWVSHHHPAVLNGQHPDTHHPGLSHSYMDAAQYPLPEEVDVLFNIDGQGNHVPPYYGNSVRATVQRYPPTHHGSQVCRPPLLHGSLPWLDGGKALGSHHTASPWNLSPFSKTSIHHGSPGPLSVYPPASSSSLSGGHASPHLFTFPPTPPKDVSPDPSLSTPGSAGSARQDEKECLKYQVPLPDSMKLESSH
Gene ID
Swiss Prot
Synonyms	HDR; HDRS; GATA3
Calculated MW	48kDa
Observed MW	48kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:6000 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. ChIP 5μg antibody for 10μg-15μg of Chromatin ChIP-seq 1:50 - 1:100
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunoprecipitation
Positive samples	SH-SY5Y, MCF7
Cellular location	Nucleus.
Customer validation	WB(Homo sapiens, Mus musculus) IHC(Mus musculus)