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GDF15 Rabbit mAb (A22097)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - GDF15 Rabbit mAb (A22097)

Western blot analysis of lysates from HT-1080 cells, using GDF15 Rabbit mAb (A22097) at1:2000 dilution.HT-1080 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

Overview

Product nameGDF15 Rabbit mAb
Catalog No.A22097
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC55565
This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. The protein is expressed in a broad range of cell types, acts as a pleiotropic cytokine and is involved in the stress response program of cells after cellular injury. Increased protein levels are associated with disease states such as tissue hypoxia, inflammation, acute injury and oxidative stress.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 100-200 of human GDF15 (NP_004855.2).
SequenceHLHLRISRAALPEGLPEASRLHRALFRLSPTASRSWDVTRPLRRQLSLARPQAPALHLRLSPPPSQSDQLLAESSSARPQLELHLRPQAARGRRRARARNG
Gene ID
Swiss Prot
SynonymsPDF; MIC1; PLAB; MIC-1; NAG-1; PTGFB; GDF-15; GDF15
Calculated MW34kDa
Observed MW15kDa/35kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHT-1080 treated by TPA
Cellular locationSecreted

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ABclonal:Western blot - GDF15 Rabbit mAb (A22097)}

Western blot - GDF15 Rabbit mAb (A22097)

Western blot analysis of lysates from HT-1080 cells, using GDF15 Rabbit mAb (A22097) at1:2000 dilution.HT-1080 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

* For research use only. Not for therapeutic or diagnostic purposes.

ELISA 키트 (1)

재조합 단백질 (2)

항체 (1)

Secondary Antibodies (26)