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GFPT1 Rabbit mAb (A20873)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GFPT1 Rabbit mAb (A20873)

Western blot analysis of lysates from Mouse large intestine, using GFPT1 Rabbit mAb (A20873) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - GFPT1 Rabbit mAb (A20873)

Western blot analysis of various lysates using GFPT1 Rabbit mAb (A20873) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - GFPT1 Rabbit mAb (A20873)

Western blot analysis of various lysates using GFPT1 Rabbit mAb (A20873) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunohistochemistry - GFPT1 Rabbit mAb (A20873)

Immunohistochemistry analysis of GFPT1 in paraffin-embedded rat colon tissue using GFPT1 Rabbit mAb (A20873) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GFPT1 Rabbit mAb (A20873)

Immunohistochemistry analysis of GFPT1 in paraffin-embedded rat tonsil tissue using GFPT1 Rabbit mAb (A20873) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GFPT1 Rabbit mAb (A20873)

Immunohistochemistry analysis of GFPT1 in paraffin-embedded human colon tissue using GFPT1 Rabbit mAb (A20873) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product nameGFPT1 Rabbit mAb
Catalog No.A20873
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2814
This gene encodes the first and rate-limiting enzyme of the hexosamine pathway and controls the flux of glucose into the hexosamine pathway. The product of this gene catalyzes the formation of glucosamine 6-phosphate.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 600-699 of human GFPT1 (Q06210).
SequenceLVDKLMPVIMIIMRDHTYAKCQNALQQVVARQGRPVVICDKEDTETIKNTKRTIKVPHSVDCLQGILSVIPLQLLAFHLAVLRGYDVDFPRNLAKSVTVE
Gene ID
Swiss Prot
SynonymsGFA; GFAT; GFPT; MSLG; CMS12; GFAT1; CMSTA1; GFAT 1; GFAT1m; GFPT1L; GFPT1
Calculated MW79kDa
Observed MW80kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, RD, Mouse large intestine, Rat testis, Rat spleen, Rat lung
Cellular locationCytosol, Extracellular exosome
Customer validation

WB(Arabidopsis thaliana)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - GFPT1 Rabbit mAb (A20873)}

    Western blot - GFPT1 Rabbit mAb (A20873)

    Western blot analysis of lysates from Mouse large intestine, using GFPT1 Rabbit mAb (A20873) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Western blot - GFPT1 Rabbit mAb (A20873)}

    Western blot - GFPT1 Rabbit mAb (A20873)

    Western blot analysis of various lysates using GFPT1 Rabbit mAb (A20873) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Western blot - GFPT1 Rabbit mAb (A20873)}

    Western blot - GFPT1 Rabbit mAb (A20873)

    Western blot analysis of various lysates using GFPT1 Rabbit mAb (A20873) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 90s.
    ABclonal:Immunohistochemistry - GFPT1 Rabbit mAb (A20873)}

    Immunohistochemistry - GFPT1 Rabbit mAb (A20873)

    Immunohistochemistry analysis of GFPT1 in paraffin-embedded rat colon tissue using GFPT1 Rabbit mAb (A20873) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - GFPT1 Rabbit mAb (A20873)}

    Immunohistochemistry - GFPT1 Rabbit mAb (A20873)

    Immunohistochemistry analysis of GFPT1 in paraffin-embedded rat tonsil tissue using GFPT1 Rabbit mAb (A20873) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - GFPT1 Rabbit mAb (A20873)}

    Immunohistochemistry - GFPT1 Rabbit mAb (A20873)

    Immunohistochemistry analysis of GFPT1 in paraffin-embedded human colon tissue using GFPT1 Rabbit mAb (A20873) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A20873? Please let us know so that we can cite the reference in this datasheet.

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