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GFPT2 Rabbit pAb (A15374)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GFPT2 Rabbit pAb (A15374)

Western blot analysis of extracts of various cell lines, using GFPT2 antibody (A15374) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunofluorescence - GFPT2 Rabbit pAb (A15374)

Immunofluorescence analysis of L929 cells using GFPT2 antibody (A15374) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameGFPT2 Rabbit pAb
Catalog No.A15374
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable glutamine-fructose-6-phosphate transaminase (isomerizing) activity. Predicted to be involved in UDP-N-acetylglucosamine metabolic process; fructose 6-phosphate metabolic process; and protein N-linked glycosylation. Predicted to act upstream of or within cellular response to leukemia inhibitory factor. Predicted to be located in cytosol. Implicated in type 2 diabetes mellitus.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 200-260 of human GFPT2 (NP_005101.1).
SequenceRGSPLLIGVRSKYKLSTEQIPILYRTCTLENVKNICKTRMKRLDSSACLHAVGDKAVEFFF
Gene ID
Swiss Prot
SynonymsGFAT; GFAT2; GFAT 2
Calculated MW77kDa
Observed MW77kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples293T, HeLa, A375, NIH/3T3, Mouse heart, Mouse pancreas, Mouse testis, Rat heart
Cellular locationcytosol
Customer validation

ChIP(Rosa hybrid cultivar)

WB(Homo sapiens)

Documents

Certificate of Compliance

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    ABclonal:Western blot - GFPT2 Rabbit pAb (A15374)}

    Western blot - GFPT2 Rabbit pAb (A15374)

    Western blot analysis of extracts of various cell lines, using GFPT2 antibody (A15374) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3s.
    ABclonal:Immunofluorescence - GFPT2 Rabbit pAb (A15374)}

    Immunofluorescence - GFPT2 Rabbit pAb (A15374)

    Immunofluorescence analysis of L929 cells using GFPT2 antibody (A15374) at dilution of 1:100. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A15374? Please let us know so that we can cite the reference in this datasheet.