제품 > 항체 > 다중클론항체(pAb)

GHD7 Rabbit pAb (A20327)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Oryza sativa

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using GHD7 Rabbit pAb (A20327) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

Overview

Product name	GHD7 Rabbit pAb
Catalog No.	A20327
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Probable transcription factor involved in the regulation of flowering time under long day (LD conditions. Plays a major role as repressor of flowering. Controls flowering time by negatively regulating the expression of EHD1 and HD3A.

Immunogen information

Immunogen	Recombinant protein of Oryza sativa GHD7.
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	Ghd7; GHD7
Calculated MW	27kDa
Observed MW	27kDa

Applications

Reactivity	Oryza sativa
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	young leaves (10 d), leaves sheath (before flower)
Cellular location	nucleus