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GKN1 Rabbit pAb (A13107)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Western blot - GKN1 Rabbit pAb (A13107)

Western blot analysis of lysates from mouse stomach, using GKN1 Rabbit pAb (A13107) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameGKN1 Rabbit pAb
Catalog No.A13107
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is found to be down-regulated in human gastric cancer tissue as compared to normal gastric mucosa.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 25-185 of human GKN1 (NP_062563.3).
SequenceLGVFLAPALANYNINVNDDNNNAGSGQQSVSVNNEHNVANVDNNNGWDSWNSIWDYGNGFAATRLFQKKTCIVHKMNKEVMPSIQSLDALVKEKKLQGKGPGGPPPKGLMYSVNPNKVDDLSKFGKNIANMCRGIPTYMAEEMQEASLFFYSGTCYTTSVL
Gene ID
Swiss Prot
SynonymsFOV; CA11; AMP18; BRICD1; foveolin; GKN1
Calculated MW20kDa
Observed MW22kDa
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse stomach
Cellular locationSecreted
Customer validation

IHC(Homo sapiens)

WB(Mus musculus)

IHC(Mus musculus)

Documents

Certificate of Compliance

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    ABclonal:Western blot - GKN1 Rabbit pAb (A13107)}

    Western blot - GKN1 Rabbit pAb (A13107)

    Western blot analysis of lysates from mouse stomach, using GKN1 Rabbit pAb (A13107) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A13107? Please let us know so that we can cite the reference in this datasheet.