제품 > 항체 > 단일클론항체(mAb)

GLDC Rabbit PolymAb® (A26427PM)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GLDC Rabbit PolymAb® (A26427PM)

Western blot analysis of various lysates using GLDC Rabbit PolymAb® (A26427PM) at 1:25000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - GLDC Rabbit PolymAb® (A26427PM)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using GLDC Rabbit PolymAb® (A26427PM) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GLDC Rabbit PolymAb® (A26427PM)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using GLDC Rabbit PolymAb® (A26427PM) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GLDC Rabbit PolymAb® (A26427PM)

Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using GLDC Rabbit PolymAb® (A26427PM) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GLDC Rabbit PolymAb® (A26427PM)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using GLDC Rabbit PolymAb® (A26427PM) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - GLDC Rabbit PolymAb® (A26427PM)

Confocal imaging of Hep G2 cells using GLDC Rabbit PolymAb® (A26427PM, dilution 1:2000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - GLDC Rabbit PolymAb® (A26427PM)

Confocal imaging of NIH/3T3 cells using GLDC Rabbit PolymAb® (A26427PM, dilution 1:2000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - GLDC Rabbit PolymAb® (A26427PM)

Confocal imaging of C6 cells using GLDC Rabbit PolymAb® (A26427PM, dilution 1:2000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product nameGLDC Rabbit PolymAb®
Catalog No.A26427PM
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Degradation of glycine is brought about by the glycine cleavage system, which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). The protein encoded by this gene is the P protein, which binds to glycine and enables the methylamine group from glycine to be transferred to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 580-749 of human GLDC (NP_000161.2).
SequenceHPFVPLDQAQGYQQLFRELEKDLCELTGYDQVCFQPNSGAQGEYAGLATIRAYLNQKGEGHRTVCLIPKSAHGTNPASAHMAGMKIQPVEVDKYGNIDAVHLKAMVDKHKENLAAIMITYPSTNGVFEENISDVCDLIHQHGGQVYLDGANMNAQVGICRPGDFGSDVSH
Gene ID
Swiss Prot
SynonymsGCE; GCSP; HYGN1
Calculated MW113kDa
Observed MW113kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:25000 - 1:150000
  • IHC-P 1:4000 - 1:16000
  • IF/ICC 1:2000 - 1:8000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesBEWO, Mouse kidney
Cellular locationMitochondrion.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - GLDC Rabbit PolymAb® (A26427PM)}

Western blot - GLDC Rabbit PolymAb® (A26427PM)

Western blot analysis of various lysates using GLDC Rabbit PolymAb® (A26427PM) at 1:25000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - GLDC Rabbit PolymAb® (A26427PM)}

Immunohistochemistry - GLDC Rabbit PolymAb® (A26427PM)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using GLDC Rabbit PolymAb® (A26427PM) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - GLDC Rabbit PolymAb® (A26427PM)}

Immunohistochemistry - GLDC Rabbit PolymAb® (A26427PM)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using GLDC Rabbit PolymAb® (A26427PM) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - GLDC Rabbit PolymAb® (A26427PM)}

Immunohistochemistry - GLDC Rabbit PolymAb® (A26427PM)

Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using GLDC Rabbit PolymAb® (A26427PM) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - GLDC Rabbit PolymAb® (A26427PM)}

Immunohistochemistry - GLDC Rabbit PolymAb® (A26427PM)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using GLDC Rabbit PolymAb® (A26427PM) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
ABclonal:Immunofluorescence - GLDC Rabbit PolymAb® (A26427PM)}

Immunofluorescence - GLDC Rabbit PolymAb® (A26427PM)

Confocal imaging of Hep G2 cells using GLDC Rabbit PolymAb® (A26427PM, dilution 1:2000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - GLDC Rabbit PolymAb® (A26427PM)}

Immunofluorescence - GLDC Rabbit PolymAb® (A26427PM)

Confocal imaging of NIH/3T3 cells using GLDC Rabbit PolymAb® (A26427PM, dilution 1:2000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - GLDC Rabbit PolymAb® (A26427PM)}

Immunofluorescence - GLDC Rabbit PolymAb® (A26427PM)

Confocal imaging of C6 cells using GLDC Rabbit PolymAb® (A26427PM, dilution 1:2000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (3)

Secondary Antibodies (26)