제품 > 항체 > 다중클론항체(pAb)

GLS2 Rabbit pAb (A16029)

Publication (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GLS2 Rabbit pAb (A16029)

Western blot analysis of extracts of various cell lines, using GLS2 antibody (A16029) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - GLS2 Rabbit pAb (A16029)

Immunofluorescence analysis of U2OS cells using GLS2 Rabbit pAb (A16029) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameGLS2 Rabbit pAb
Catalog No.A16029
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Originally thought to be liver-specific, this protein has been found in other tissues as well. Alternative splicing results in multiple transcript variants that encode different isoforms.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 545-602 of human GLS2 (NP_037399.2).
SequenceNPFAKDRWGNIPLDDAVQFNHLEVVKLLQDYQDSYTLSETQAEAAAEALSKENLESMV
Gene ID
Swiss Prot
SynonymsGA; GLS; LGA; hLGA
Calculated MW66kDa
Observed MW66kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse kidney, Rat kidney
Cellular locationMitochondrion
Customer validation

WB(Mus musculus, Rattus norvegicus)

Other(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - GLS2 Rabbit pAb (A16029)}

Western blot - GLS2 Rabbit pAb (A16029)

Western blot analysis of extracts of various cell lines, using GLS2 antibody (A16029) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - GLS2 Rabbit pAb (A16029)}

Immunofluorescence - GLS2 Rabbit pAb (A16029)

Immunofluorescence analysis of U2OS cells using GLS2 Rabbit pAb (A16029) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A16029? Please let us know so that we can cite the reference in this datasheet.