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GLUD1 Rabbit mAb (A5176)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:GLUD1 Rabbit mAb
ABclonal:Western blot - GLUD1 Rabbit mAb (A5176)

Western blot analysis of various lysates using GLUD1 Rabbit mAb (A5176) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - GLUD1 Rabbit mAb (A5176)

Western blot analysis of various lysates using GLUD1 Rabbit mAb (A5176) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - GLUD1 Rabbit mAb (A5176)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using GLUD1 Rabbit mAb (A5176) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GLUD1 Rabbit mAb (A5176)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using GLUD1 Rabbit mAb (A5176) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GLUD1 Rabbit mAb (A5176)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using GLUD1 Rabbit mAb (A5176) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GLUD1 Rabbit mAb (A5176)

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using GLUD1 Rabbit mAb (A5176) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GLUD1 Rabbit mAb (A5176)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using GLUD1 Rabbit mAb (A5176) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GLUD1 Rabbit mAb (A5176)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using GLUD1 Rabbit mAb (A5176) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GLUD1 Rabbit mAb (A5176)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using GLUD1 Rabbit mAb (A5176) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GLUD1 Rabbit mAb (A5176)

Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using GLUD1 Rabbit mAb (A5176) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - GLUD1 Rabbit mAb (A5176)

Immunofluorescence analysis of NIH-3T3 cells using GLUD1 Rabbit mAb (A5176) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameGLUD1 Rabbit mAb
Catalog No.A5176
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1216
This gene encodes glutamate dehydrogenase, which is a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid-induced insulin secretion. It is allosterically activated by ADP and inhibited by GTP and ATP. Activating mutations in this gene are a common cause of congenital hyperinsulinism. Alternative splicing of this gene results in multiple transcript variants. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Related pseudogenes have been identified on chromosomes 10, 18 and X.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 459-558 of human GLUD1 (P00367).
SequenceERDSNYHLLMSVQESLERKFGKHGGTIPIVPTAEFQDRISGASEKDIVHSGLAYTMERSARQIMRTAMKYNLGLDLRTAAYVNAIEKVFKVYNEAGVTFT
Gene ID
Swiss Prot
SynonymsGDH; GDH1; GLUD; hGDH1; GLUD1
Calculated MW61kDa
Observed MW52kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:4000
  • IHC-P 1:200 - 1:2000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesJurkat, K-562, U-251MG, Mouse liver, Rat liver
Cellular locationEndoplasmic reticulum, Mitochondrion.
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - GLUD1 Rabbit mAb (A5176)}

Western blot - GLUD1 Rabbit mAb (A5176)

Western blot analysis of various lysates using GLUD1 Rabbit mAb (A5176) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - GLUD1 Rabbit mAb (A5176)}

Western blot - GLUD1 Rabbit mAb (A5176)

Western blot analysis of various lysates using GLUD1 Rabbit mAb (A5176) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - GLUD1 Rabbit mAb (A5176)}

Immunohistochemistry - GLUD1 Rabbit mAb (A5176)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using GLUD1 Rabbit mAb (A5176) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - GLUD1 Rabbit mAb (A5176)}

Immunohistochemistry - GLUD1 Rabbit mAb (A5176)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using GLUD1 Rabbit mAb (A5176) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - GLUD1 Rabbit mAb (A5176)}

Immunohistochemistry - GLUD1 Rabbit mAb (A5176)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using GLUD1 Rabbit mAb (A5176) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - GLUD1 Rabbit mAb (A5176)}

Immunohistochemistry - GLUD1 Rabbit mAb (A5176)

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using GLUD1 Rabbit mAb (A5176) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - GLUD1 Rabbit mAb (A5176)}

Immunohistochemistry - GLUD1 Rabbit mAb (A5176)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using GLUD1 Rabbit mAb (A5176) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - GLUD1 Rabbit mAb (A5176)}

Immunohistochemistry - GLUD1 Rabbit mAb (A5176)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using GLUD1 Rabbit mAb (A5176) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - GLUD1 Rabbit mAb (A5176)}

Immunohistochemistry - GLUD1 Rabbit mAb (A5176)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using GLUD1 Rabbit mAb (A5176) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - GLUD1 Rabbit mAb (A5176)}

Immunohistochemistry - GLUD1 Rabbit mAb (A5176)

Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using GLUD1 Rabbit mAb (A5176) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunofluorescence - GLUD1 Rabbit mAb (A5176)}

Immunofluorescence - GLUD1 Rabbit mAb (A5176)

Immunofluorescence analysis of NIH-3T3 cells using GLUD1 Rabbit mAb (A5176) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

Secondary Antibodies (26)