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GNB2 Rabbit mAb (A9643)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GNB2 Rabbit mAb (A9643)

Western blot analysis of various lysates using GNB2 Rabbit mAb (A9643) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - GNB2 Rabbit mAb (A9643)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using GNB2 Rabbit mAb (A9643) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Overview

Product nameGNB2 Rabbit mAb
Catalog No.A9643
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1675
Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. This gene contains a trinucleotide (CCG) repeat length polymorphism in its 5' UTR.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human GNB2 (P62879).
SequenceMSELEQLRQEAEQLRNQIRDARKACGDSTLTQITAGLDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKV
Gene ID
Swiss Prot
SynonymsSSS4; HG2C1; NEDHYDF; GNB2
Calculated MW37kDa
Observed MW35kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, NIH/3T3, Jurkat, SH-SY5Y, Rat brain
Cellular locationCytoplasm, perinuclear region, Cell membrane
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - GNB2 Rabbit mAb (A9643)}

Western blot - GNB2 Rabbit mAb (A9643)

Western blot analysis of various lysates using GNB2 Rabbit mAb (A9643) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - GNB2 Rabbit mAb (A9643)}

Immunohistochemistry - GNB2 Rabbit mAb (A9643)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using GNB2 Rabbit mAb (A9643) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

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