제품 > 항체 > 다중클론항체(pAb)

GPR108 Rabbit pAb (A16554)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GPR108 Rabbit pAb (A16554)

Western blot analysis of various lysates, using GPR108 Rabbit pAb (A16554) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 25s.

Overview

Product nameGPR108 Rabbit pAb
Catalog No.A16554
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to be involved in negative regulation of toll-like receptor signaling pathway. Located in trans-Golgi network.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 33-260 of human GPR108 (NP_001073921.1).
SequenceRIHQLALTGEKRADIQLNSFGFYTNGSLEVELSVLRLGLREAEEKSLLVGFSLSRVRSGRVRSYSTRDFQDCPLQKNSSSFLVLFLINTKDLQVQVRKYGEQKTLFIFPGLLPEAPSKPGLPKPQATVPRKVDGGGTSAASKPKSTPAVIQGPSGKDKDLVLGLSHLNNSYNFSFHVVIGSQAEEGQYSLNFHNCNNSVPGKEHPFDITVMIREKNPDGFLSAAEMPL
Gene ID
Swiss Prot
SynonymsLUSTR2; GPR108
Calculated MW61kDa
Observed MW76kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesU-87MG, U2OS, Mouse colon, Rat thymus
Cellular locationMembrane, Multi-pass membrane protein.

Documents

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ABclonal:Western blot - GPR108 Rabbit pAb (A16554)}

Western blot - GPR108 Rabbit pAb (A16554)

Western blot analysis of various lysates, using GPR108 Rabbit pAb (A16554) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 25s.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)