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GPX7 Rabbit pAb (A3902)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GPX7 Rabbit pAb (A3902)

Western blot analysis of extracts of various cell lines, using GPX7 antibody (A3902) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameGPX7 Rabbit pAb
Catalog No.A3902
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables catalase activity. Predicted to be involved in cellular response to oxidative stress. Located in endoplasmic reticulum.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 20-187 of human GPX7 (NP_056511.2).
SequenceQQEQDFYDFKAVNIRGKLVSLEKYRGSVSLVVNVASECGFTDQHYRALQQLQRDLGPHHFNVLAFPCNQFGQQEPDSNKEIESFARRTYSVSFPMFSKIAVTGTGAHPAFKYLAQTSGKEPTWNFWKYLVAPDGKVVGAWDPTVSVEEVRPQITALVRKLILLKREDL
Gene ID
Swiss Prot
SynonymsGPX6; CL683; GPx-7; NPGPx; GSHPx-7
Calculated MW21kDa
Observed MW21kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesU-87MG, LO2, SW480, Mouse brain, Mouse heart
Cellular locationSecreted
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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    ABclonal:Western blot - GPX7 Rabbit pAb (A3902)}

    Western blot - GPX7 Rabbit pAb (A3902)

    Western blot analysis of extracts of various cell lines, using GPX7 antibody (A3902) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A3902? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)