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GSTM2 Rabbit pAb (A13496)

Review (1)Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - GSTM2 Rabbit pAb (A13496)

Western blot analysis of extracts of mouse testis, using GSTM2 antibody (A13496) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Review
ABclonal: review for GSTM2 Rabbit pAb(A13496)

Overview

Product nameGSTM2 Rabbit pAb
Catalog No.A13496
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-218 of human GSTM2 (NP_000839.1).
SequenceMPMTLGYWNIRGLAHSIRLLLEYTDSSYEEKKYTMGDAPDYDRSQWLNEKFKLGLDFPNLPYLIDGTHKITQSNAILRYIARKHNLCGESEKEQIREDILENQFMDSRMQLAKLCYDPDFEKLKPEYLQALPEMLKLYSQFLGKQPWFLGDKITFVDFIAYDVLERNQVFEPSCLDAFPNLKDFISRFEGLEKISAYMKSSRFLPRPVFTKMAVWGNK
Gene ID
Swiss Prot
SynonymsGST4; GSTM; GTHMUS; GSTM2-2
Calculated MW26kDa
Observed MW27kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse testis
Cellular locationCytoplasm
Customer validation

(muscles tissue)

WB(Rattus norvegicus, Mus musculus)

IF(Mus musculus)

Documents

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ABclonal:Western blot - GSTM2 Rabbit pAb (A13496)}

Western blot - GSTM2 Rabbit pAb (A13496)

Western blot analysis of extracts of mouse testis, using GSTM2 antibody (A13496) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)